Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Linear optics based nanoscopy.
TL;DR: A novel method for observing sub-wavelength features in a conventional optical microscope using linear optics based on a random and time varying flow of nanoparticles moving in proximity to the inspected sample is presented.
Journal ArticleDOI
New observations in neuroscience using superresolution microscopy
Michihiro Igarashi,Motohiro Nozumi,Ling-Gang Wu,Francesca Cella Zanacchi,István Katona,László Barna,Pingyong Xu,Mingshu Zhang,Fudong Xue,Edward S. Boyden +9 more
TL;DR: Using super-resolution microscopy techniques, experts in these different types of SM discuss the new structural and functional information about specific important molecules in neuroscience that has been gained with SM, and reveal novel mechanisms of endocytosis in nerve growth, fusion pore dynamics, and quantitative new properties of excitatory and inhibitory synapses.
Journal ArticleDOI
A survey on applications of deep learning in microscopy image analysis.
TL;DR: In this article, a review article introduces the applications of deep learning algorithms in microscopy image analysis, which include image classification, region segmentation, object tracking and super-resolution reconstruction, and discuss the drawbacks of existing deep learning-based methods, especially on the challenges of training datasets acquisition and evaluation, and propose the potential solutions.
Journal ArticleDOI
Review of advanced imaging techniques
TL;DR: This paper reviews several recently developed microscopic techniques, including diffraction-limited methods (e.g., confocal microscopy, 2-photon microscope, 4Pi microscopy), and spatially modulated illumination microscopy and subdiffraction techniques ( e.g, photoactivated localization microscopy) and highlights the fundamentals and applications of advanced optical imaging techniques.
Journal ArticleDOI
Fluorescent nuclear track detectors – Review of past, present and future of the technology
TL;DR: Fluorescent Nuclear Track Detector (FNTD) as mentioned in this paper is a passive luminescent integrating detector technology having important advantages in measuring neutrons, heavy ions and even photons, it is based on new aluminum oxide crystals doped with carbon and magnesium impurities (Al2O3:C,Mg) and confocal laser scanning fluorescent microscopy technique.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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