Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
On-Chip Super-Resolution Imaging with Fluorescent Polymer Films
Chenlei Pang,Jingxi Li,Mingwei Tang,Jianpu Wang,Ioanna Mela,Florian Ströhl,Lisa Hecker,Weidong Shen,Qiulan Liu,Xiaowei Liu,Yinan Wang,Hao Zhang,Meng Xu,Xinghong Zhang,Xu Liu,Qing Yang,Qing Yang,Clemens F. Kaminski +17 more
TL;DR: In this paper, a super-resolution chip (SRC) is demonstrated using a specially designed waveguide chip that can illuminate a sample with multicolor evanescent waves travelling along different directions.
Patent
Apparatus for selective excitation of microparticles
TL;DR: In this article, an interference pattern generation module was proposed to generate an excitation pattern for nucleic acid microparticles by splitting a first laser beam into second and third laser beams.
Journal ArticleDOI
Rotationally reconfigurable metamaterials based on moiré phenomenon
Jae-Hyung Han,Inbo Kim,Jung-Wan Ryu,Jungjoon Kim,Jin-Ho Cho,Geo-Su Yim,Hyunsung Park,Bumki Min,Muhan Choi +8 more
TL;DR: It is shown that the transmission of electromagnetic waves can be controlled from 90% to 10% at 11 GHz by experiments and numerical simulation and the reconfigurable metamaterial proposed here can be applied in bandpass filters and tunable modulation devices.
Journal ArticleDOI
Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy
Ian L. Gunsolus,Dehong Hu,Cosmin Mihai,Samuel E. Lohse,Chang-Soo Lee,Marco D. Torelli,Robert J. Hamers,Catherine J. Murhpy,Galya Orr,Christy L. Haynes +9 more
TL;DR: It is shown that this staining method may be used to resolve the bacterial cell surface with sub-diffraction-limited resolution and to identify localization patterns of nanomaterials, specifically cadmium selenide quantum dots, following interaction with bacterial cells.
Journal ArticleDOI
Nonredundant Raman imaging using optical eigenmodes
S. Kosmeier,Svetlana Zolotovskaya,Anna Chiara De Luca,Andrew Riches,C. Simon Herrington,Kishan Dholakia,Michael Mazilu +6 more
TL;DR: In this article, the authors demonstrate the application of optical eigenmodes (OEis) to wide field, scan-free spontaneous Raman imaging, which is notoriously slow in wide-field mode.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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