Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Any Way You Slice It-A Comparison of Confocal Microscopy Techniques.
James Jonkman,Claire M. Brown +1 more
TL;DR: The grid confocal, classic confocal laser-scanning microscope (CLSM), the resonant scanning-CLSM, and the spinning-disk confocal microscope are covered.
Journal ArticleDOI
DNA-barcoded labeling probes for highly multiplexed Exchange-PAINT imaging
Sarit S. Agasti,Sarit S. Agasti,Sarit S. Agasti,Yu Wang,Yu Wang,Florian Schueder,Aishwarya Sukumar,Ralf Jungmann,Peng Yin,Peng Yin +9 more
TL;DR: This work reports the development of multiplexed cellular super-resolution imaging using DNA-barcoded binders and its applications in medicine, materials science and nano-materials.
Patent
Method and apparatus for imaging of vessel segments
TL;DR: In this paper, an apparatus, method and software arrangement for imaging a surface of a structure that is in contact with an opaque fluid is provided, which includes an article of manufacture (e.g., a housing), a fluid delivery arrangement and an imaging arrangement.
Journal ArticleDOI
Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison.
Eva Wegel,Antonia Gohler,B C Lagerholm,Alan Wainman,Stephan Uphoff,Rainer Kaufmann,Rainer Kaufmann,Ian M. Dobbie +7 more
TL;DR: In this article, the authors compare the individual strengths and weaknesses of SIM, STED, and SMLM by imaging a variety of different subcellular structures in fixed cells.
Journal ArticleDOI
Structured illumination microscopy: artefact analysis and reduction utilizing a parameter optimization approach
TL;DR: This work investigated some significant causes of artefacts in structured illumination microscopy, and developed a correction approach that can be applied to images after acquisition that produces exceptional, artefact‐free results in everyday laboratory work.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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