Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Book ChapterDOI
Breaking the resolution limit in light microscopy.
Rainer Heintzmann,Gabriella Ficz +1 more
TL;DR: This chapter describes some of these methods and how they break the classical resolution limit in fluorescence microscopy, and the most promising approaches are all based on fluorescence and use either nonlinear interaction of light with the sample or precise localization of individual particles or molecules with subsequent image generation.
Journal ArticleDOI
Advanced microscopy methods for visualizing chromatin structure.
TL;DR: Thanks to the advent of super‐resolution microscopy methods, the authors are finally starting to be able to probe the nanoscale organization of chromatin in vivo and these methods have the potential to greatly advance the knowledge about chromatin structure and function relationship.
Journal ArticleDOI
Super-resolution scanning laser microscopy through virtually structured detection.
TL;DR: A super-resolution scanning laser imaging system which employs virtually structured detection (VSD) to break the diffraction limit and provides an easy, low-cost and phase-artifact free strategy to achieve super- resolution in scanning laser microscopy.
Journal ArticleDOI
Recent advancements in structured-illumination microscopy toward live-cell imaging.
TL;DR: Structured-illumination microscopy (SIM), a type of super-resolution microscopy, doubles the spatial resolution in all three dimensions by illuminating the sample with a patterned excitation light, followed by computer reconstruction.
Journal ArticleDOI
Wide-angle speckleless DMD holographic display using structured illumination with temporal multiplexing
TL;DR: A digital micromirror device (DMD) holographic display, where speckleless holograms can be observed in the expanded viewing zone through temporal multiplexing and utilizing an active filter array for the Fourier filter and synchronize it with the LD array.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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