Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Double-label confocal laser-scanning microscopy, image restoration, and real-time three-dimensional reconstruction to study axons in the central nervous system and their contacts with target neurons.
Floris G. Wouterlood,Theo van Haeften,Nico Blijleven,Pepa Pérez-Templado,Helena Pérez-Templado +4 more
TL;DR: Rotation on the computer of the three-dimensional reconstructed fibers and dendrites along all three spatial axes enabled the authors to determine whether “true” or “false” contacts occur, and to measure the actual twin image mismatch of the confocal instrument.
Proceedings ArticleDOI
OTF compensation in structured illumination superresolution images
TL;DR: In this paper, a de-aliased Fourier space is used to separate the conventional, unaliased object spatial frequencies from the aliased ones, which contain superresolution information.
Journal ArticleDOI
Enhancement of fluorescence confocal scanning microscopy lateral resolution by use of structured illumination
TL;DR: In this article, a structured illumination confocal scanning microscopy (SICSM) was proposed to improve the lateral resolution of the confocal microscope by using structured illumination to improve axial resolution.
Journal ArticleDOI
Bessel terahertz imaging with enhanced contrast realized by silicon multi-phase diffractive optics.
Linas Minkevičius,Domas Jokubauskis,Irmantas Kašalynas,Sergej Orlov,Antanas Urbas,Gintaras Valušis +5 more
TL;DR: A proposed Bessel zone plate (BZP) design - discrete axicon containing 4 phase quantization levels - based on high-resistivity silicon and produced by laser ablation technology allowed to extend the focal depth up to 20 mm with minimal optical losses and refuse employment of bulky parabolic mirrors in the imaging setup.
Journal ArticleDOI
Polarization-Controlled Plasmonic Structured Illumination
TL;DR: A polarization tunable structured plasmonic fields (SPF) is proposed and experimentally demonstrated that can be continuously shifted by merely varying the linear polarization state of an incident beam.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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