Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
More filters
Journal ArticleDOI
High loop rate adaptive optics flood illumination ophthalmoscope with structured illumination capability.
Elena Gofas-Salas,Pedro Mecê,C. Petit,Jessica Jarosz,Laurent M. Mugnier,Aurélie Bonnefois,Kate Grieve,Jose A. Sahel,Michel Paques,Serge Meimon +9 more
TL;DR: The design and performance of an adaptive optics flood illumination ophthalmoscope (AO-FIO) platform, based on eye motion and dynamic aberrations experimental analysis, that opens up the prospect of a return to favor of flood illumination adaptive optics systems provided that its high pixel rate and structured illumination capabilities are exploited.
Journal ArticleDOI
Resolution enhancement of saturated fluorescence emission difference microscopy.
TL;DR: Simulations based on the saturated model of rhodamine 6G, as well as experiments on biological samples, are presented to verify the capability of the proposed concept and show the unprecedented resolving ability of the saturated FED method.
Journal ArticleDOI
Two-photon image-scanning microscopy with SPAD array and blind image reconstruction.
Sami Koho,Eli Slenders,Giorgio Tortarolo,Marco Castello,Mauro Buttafava,Federica Villa,Elena Tcarenkova,Marcel Ameloot,Paolo Bianchini,Colin J. R. Sheppard,Alberto Diaspro,Alberto Tosi,Giuseppe Vicidomini +12 more
TL;DR: A straightforward implementation of 2PE image scanning microscopy (2PE-ISM) that, by leveraging the authors' recently introduced single-photon avalanche diode (SPAD) array detector and a novel blind image reconstruction method, is shown to enhance the effective resolution, as well as the overall image quality of 2 PE microscopy.
Journal ArticleDOI
A framework for far-field infrared absorption microscopy beyond the diffraction limit.
TL;DR: A framework is proposed for infrared (IR) absorption microscopy in the far-field with a spatial resolution below the diffraction limit and a resolution of 250 nm was found when probing the CH₂ stretches at 3.5 μm with pump energies less than ten times the vibrational saturation threshold.
Dissertation
Structured Laser Illumination Planar Imaging SLIPI Applications for Spray Diagnostics
TL;DR: Structured Laser Illumination Planar Imaging (SLIPI) as discussed by the authors is a 2D imaging technique based on planar laser imaging that uses a sophisticated illumination scheme - spatial intensity modulation - to differentiate between the intensity contribution arising from directly and multiply scattered light.
References
More filters
BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
Related Papers (5)
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more