Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Structured illumination microscopy using a photonic chip
Øystein Ivar Helle,Firehun Tsige Dullo,Marcel Lahrberg,Jean-Claude Tinguely,Balpreet Singh Ahluwalia +4 more
TL;DR: In this article, a photonic-chip-based total internal reflection fluorescence (TIRF)-SIM was proposed to reduce the complexity of the optical setup needed to acquire TIRF-SIM images.
Journal ArticleDOI
A mathematical theory of super-resolution by using a system of sub-wavelength Helmholtz resonators ∗
Habib Ammari,Hai Zhang +1 more
TL;DR: Lemoult et al. as discussed by the authors developed a rigorous mathematical theory to explain the super-resolution phenomenon observed in the experiment and provided an elegant and systematic way for calculating resonant frequencies for Helmholtz resonators in assorted space settings, as well as in various frequency regimes.
Journal ArticleDOI
High-speed atomic force microscopy combined with inverted optical microscopy for studying cellular events
Yuki Suzuki,Nobuaki Sakai,Aiko Yoshida,Yoshitsugu Uekusa,Akira Yagi,Yuka Imaoka,Shuichi Ito,Koichi Karaki,Kunio Takeyasu +8 more
TL;DR: This work describes the implementation of high-speed AFM coupled with an optical fluorescence microscope by developing a tip-scanning system, instead of a sample- scanning system, which operates on an inverted optical microscope, and conducted structural studies of living HeLa and 3T3 fibroblast cell surfaces.
Journal ArticleDOI
2000-fold parallelized dual-color STED fluorescence nanoscopy
TL;DR: A dual-color STED nanoscope utilizing two orthogonally crossed standing light waves as a fluorescence switch-off pattern, and providing a resolving power down to 30 nm is presented, demonstrating the imaging capabilities in a biological context for immunostained vimentin fibers.
Journal ArticleDOI
Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques.
Anja Ostrowski,Daniel Nordmeyer,Alexander Boreham,Cornelia Holzhausen,Lars Mundhenk,Christina Graf,Martina C. Meinke,Annika Vogt,Sabrina Hadam,Jürgen Lademann,Eckart Rühl,Ulrike Alexiev,Achim D. Gruber +12 more
TL;DR: Different imaging techniques for localizing inorganic as well as organic nanoparticles in tissues, cells and subcellular compartments are described and compared and the different approaches vary in terms of applicability for specific particles, sensitivity, optical resolution, technical requirements and thus availability, and effects of labeling on particle properties.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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