Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy
TL;DR: In this article, the authors summarized the recent advances of upconversion nanoparticles for super-resolution microscopy and projected the future directions of this emerging field and highlighted the opportunities and challenges.
Journal ArticleDOI
Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction.
TL;DR: In this paper, a structured illumination (SI) microscopy is proposed to integrate the principles of fluorescent super-resolution and coherent synthetic aperture to achieve 3D dual-modality sub-diffraction resolution, fluorescence and refractive index (RI) visualizations of biological samples.
Journal ArticleDOI
Emerging applications of digital micromirror devices in biophotonic fields
TL;DR: Two types of biophotonic applications using DMDs are explored: DMD-implemented high-speed and high-resolution imaging microscopes and bioprinting and lithographic applications by freely modulated light patterns using a DMD.
Journal ArticleDOI
New ways of looking at very small holes – using optical nanoscopy to visualize liver sinusoidal endothelial cell fenestrations
Cristina Ionica Øie,Viola Mönkemöller,Wolfgang Hübner,Mark Schüttpelz,Hong Mao,Balpreet Singh Ahluwalia,Thomas R Huser,Peter McCourt +7 more
TL;DR: This review will cover the current state of the art of the use of nanoscopy in the study of liver endothelium and the liver in general and potential future applications in cell biology and the clinical implications will be discussed.
Journal ArticleDOI
High-resolution imaging of autofluorescent particles within drusen using structured illumination microscopy
Sabrina Rossberger,Thomas Ach,Gerrit Best,Christoph Cremer,Christoph Cremer,Rainer Heintzmann,Rainer Heintzmann,Stefan Dithmar +7 more
TL;DR: Sim allows studying AF particles within drusen on a higher resolution level compared with conventional fluorescence, multiphoton or even confocal microscopy and therefore provides detailed insights in Drusen.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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