Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Near-field Fourier ptychography: super-resolution phase retrieval via speckle illumination
TL;DR: In this article, a near-field Fourier ptychography (NFFP) imaging modality was proposed for high-resolution imaging in both microscopic and macroscopic imaging settings.
Journal ArticleDOI
Far-field unlabeled super-resolution imaging with superoscillatory illumination
Edward T. F. Rogers,Shmma Quraishe,Katrine S. Rogers,Tracey A. Newman,Peter K. Smith,Peter K. Smith,Nikolay I. Zheludev,Nikolay I. Zheludev +7 more
TL;DR: In this paper, the authors demonstrate microscopy with super-oscillatory illumination of the object and describe its underlying principles, and demonstrate high-frame-rate polarization contrast imaging of living cells with a resolution significantly exceeding that achievable with conventional instruments.
Journal ArticleDOI
Real-time two- and three-dimensional imaging of monocyte motility and navigation on planar surfaces and in collagen matrices: roles of Rho
Robert Bzymek,Markus Horsthemke,Katrin Isfort,Simon Mohr,Kerstin Tjaden,Carsten Müller-Tidow,Marlies Thomann,Tanja Schwerdtle,Martin Bähler,Albrecht Schwab,Peter J. Hanley +10 more
TL;DR: In addition to its role in tail retraction on 2D surfaces, Rho is critical for movement in confined spaces, but is largely redundant for motility and chemotaxis in loose matrices.
Journal ArticleDOI
A method for super-resolved CARS microscopy with structured illumination in two dimensions
TL;DR: The results demonstrate that the method promises a particular benefit on CARS microscopy by adding the super-resolution capability to improve its 2D spatial resolution by a factor of approximately three.
Journal ArticleDOI
Non-bleaching fluorescence emission difference microscopy using single 808-nm laser excited red upconversion emission.
TL;DR: By introducing Er3+ activated upconverting nanoparticles with red-color emission and non-photobleaching properties, this work demonstrates nonbleaching super-resolution imaging with FED microscopy.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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