Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Fluorescent Probes for Lipid Rafts: From Model Membranes to Living Cells
Andrey S. Klymchenko,Rémy Kreder +1 more
TL;DR: An urgent need to develop new probes, specifically adapted for cell plasma membranes and compatible with modern fluorescence microscopy techniques to push the understanding of membrane microdomains forward is highlighted.
Patent
Method and apparatus for performing optical imaging using frequency-domain interferometry
TL;DR: In this article, the first and/or second electro-magnetic radiations have a spectrum whose mean frequency changes substantially continuously over time at a tuning speed that is greater than 100 Tera Hertz per millisecond.
Journal ArticleDOI
Deep-STORM: super-resolution single-molecule microscopy by deep learning
TL;DR: Deep-STORM as mentioned in this paper uses a deep convolutional neural network that can be trained on simulated data or experimental measurements, both of which are demonstrated to achieve state-of-the-art resolution under challenging signal-to-noise conditions and high emitter densities.
Patent
System and method for optical coherence imaging
TL;DR: In this paper, a system and method for imaging of a sample, e.g., biological sample, are provided, where at least one source electro-magnetic radiation forwarded to the sample and a reference may be generated.
Journal ArticleDOI
Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution.
E. H. Rego,Lin Shao,J. J. Macklin,Lukman Winoto,G. A. Johansson,Nick Kamps-Hughes,Michael W. Davidson,Mats G. L. Gustafsson +7 more
TL;DR: In this article, the authors demonstrate that reversible photoswitching of a fluorescent protein provides the required nonlinearity at light intensities six orders of magnitude lower than those needed for saturation, and visualize cellular structures by imaging the mammalian nuclear pore and actin cytoskeleton.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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