Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Patent
Fluorescence light scanning microscope having a birefringent chromatic beam shaping device
Matthias Reuss,Johann Engelhardt +1 more
TL;DR: In this paper, a chromatic beam shaping device was used to shape a polarization distribution of the beam of suppression light over its beam cross-section such that the suppression light left a shape of the excitation light such that it had at least one intensity zero essentially at the focus point.
Proceedings ArticleDOI
Imaging interferometric microscopy
TL;DR: In this paper, the authors introduced the concept of imaging interferometric microscopy (IIM) to achieve high-resolution images corresponding to /spl sim/3 times the resolution achieved with a low-NA microscope objective.
Journal ArticleDOI
Analytical model of the optical vortex microscope.
TL;DR: An analytical solution of the whole path of the beam in the system (within paraxial approximation)-from the vortex lens to the observation plane situated on the CCD camera, and derives a simple expression for the vortex trajectory of small vortex displacements.
Journal ArticleDOI
Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation
Marco Pascucci,Sivaramankrishna Ganesan,Aditya Tripathy,Ori Katz,Valentina Emiliani,Marc Guillon +5 more
TL;DR: In this article, a compressed sensing approach was proposed to enable 3D sub-diffraction of cultured cells by saturating fluorescence excitation, exploiting the natural orthogonality of transverse speckle illumination planes, and 3D probing of the sample is achieved by a single two-dimensional scan.
Journal ArticleDOI
Quantitative structured-illumination phase microscopy.
TL;DR: A quantitative phase imaging method for homogeneous objects with a bright field transmission microscope using an amplitude mask and a digital processing algorithm to estimate the object's quantitative OPL profile based on a closed form analytical solution derived using a ray optics model for objects with small OPL gradients.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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