Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Upholding the diffraction limit in the focusing of light and sound
Alexei Maznev,Oliver B. Wright +1 more
TL;DR: The concept of the diffraction limit put forth by Ernst Abbe and others has been an important guiding principle limiting our ability to tightly focus classical waves such as light and sound, in the far field.
Journal ArticleDOI
Shh promotes direct interactions between epidermal cells and osteoblast progenitors to shape regenerated zebrafish bone.
TL;DR: In the regenerating zebrafish fin, local Hedgehog signalling between basal epidermal cells and osteoblast progenitors controls ray branching and hence skeletal patterning.
Posted Content
Convolutional neural networks that teach microscopes how to image.
TL;DR: This work increases the classification accuracy of a microscope's recorded images by merging an optical model of image formation into the pipeline of a CNN, and simultaneously determines an ideal illumination arrangement to highlight important sample features during image acquisition.
Journal ArticleDOI
Ordering of myosin II filaments driven by mechanical forces: experiments and theory.
Kinjal Dasbiswas,Shiqiong Hu,Shiqiong Hu,Frank Schnorrer,Samuel A. Safran,Alexander D. Bershadsky +5 more
TL;DR: It is proposed that the myosin II filaments embedded into elastic, intervening actin network (IVN) function as force dipoles that interact attractively through the IVN.
Journal ArticleDOI
Experimental methods of molecular matter-wave optics.
TL;DR: The state of the art in preparing, manipulating and detecting coherent molecular matter and experimental methods for handling the quantum motion of compound systems from diatomic molecules to clusters or biomolecules are described.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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