Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy
Yunqing Tang,Luru Dai,Xiaoming Zhang,Junbai Li,Johnny Hendriks,Xiaoming Fan,Nadine Gruteser,Annika Meisenberg,Arnd Baumann,Alexandros Katranidis,Thomas Gensch +10 more
TL;DR: This work presents a real-time, and robust single molecule identification and localization algorithm, SNSMIL (Shot Noise based Single Molecule Identification and Localization), based on the intrinsic nature of noise, which improves the identification accuracy of single fluorescent molecules in experimental or simulated datasets with high and inhomogeneous background.
Computational Cameras: Approaches, Benefits and Limits
TL;DR: The benefits and limits of computational imaging are explored, and how it is related to the adjacent and overlapping fields of digital imaging, computational photography and computational image sensors is discussed.
Journal ArticleDOI
Three-dimensional differential interference contrast microscopy using synthetic aperture imaging.
Moonseok Kim,Youngwoon Choi,Christopher Fang-Yen,Yongjin Sung,Kwanhyung Kim,Ramachandra R. Dasari,Michael S. Feld,Wonshik Choi +7 more
TL;DR: This work implements differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2 and obtains high-resolution and high-contrast 3-D DIC imaging of live biological cells.
Journal ArticleDOI
Resolution enhancement of digital holographic microscopy via synthetic aperture: a review
Peng Gao,Caojin Yuan +1 more
TL;DR: In this paper , the authors review various resolution enhancement approaches in digital holographic microscopy (DHM) and discuss the advantages and disadvantages of these approaches and their practical applications in many fields.
Journal ArticleDOI
Computational structured illumination for high-content fluorescence and phase microscopy.
TL;DR: In this article, the authors used laterally-translated Scotch tape to generate high-resolution speckle illumination patterns across a large FOV, and custom optimization algorithms then jointly reconstruct the sample's super-resolution fluorescent and quantitative phase distributions, while digitally correcting for system imperfections.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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