Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Structured planar laser-induced fluorescence (S-PLIF) for the accurate identification of interfaces in multiphase flows
TL;DR: In this article, the authors proposed a structured planar laser-induced fluorescence (S-PLIF) method, which can suppress the errors in PLIF-derived film thickness measurements that arise due to total internal reflection (TIR) of the emitted fluorescence at the phase boundary.
Journal ArticleDOI
Chemical Analysis of Single Cells and Organelles.
TL;DR: This paper presents Electrochemical Analysis of Single Cells and Organelles, a large-scale study of single cells and organelles using SuperResolution Microscopy for imaging, and some of the techniques used in this study, as well as some new approaches to characterization.
Journal ArticleDOI
Molecular Flow Quantified beyond the Diffraction Limit by Spatiotemporal Image Correlation of Structured Illumination Microscopy Data
TL;DR: These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form of velocity histograms and flow vector maps.
Journal ArticleDOI
Correcting for photodestruction in super-resolution optical fluctuation imaging.
Yves Peeters,Wim Vandenberg,Sam Duwé,Arno Bouwens,Arno Bouwens,Tomas Lukes,Tomas Lukes,Cyril Ruckebusch,Theo Lasser,Peter Dedecker +9 more
TL;DR: This work develops a procedure that can robustly estimate to what extent any particular experiment is affected by photodestruction and identifies two approaches that can correct for the presence of even strong photodESTruction, one of which can be implemented directly in the SOFI calculation software.
Journal ArticleDOI
On optical detection of densely labeled synapses in neuropil and mapping connectivity with combinatorially multiplexed fluorescent synaptic markers
TL;DR: It is concluded that with the described approach neural connectivity in macroscopically large neural circuits can be mapped with great accuracy, in scalable manner, using fast optical tools, and straightforward image processing.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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