Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Technological advances in super-resolution microscopy to study cellular processes
TL;DR: In this paper , the authors review the technical details behind the most common implementations of super-resolution microscopy and highlight some of the recent, promising advances in this field, and present a new window into visualizing the inner life of cells at unprecedented levels of detail.
Journal ArticleDOI
Real-time fluorescence imaging with 20 nm axial resolution
TL;DR: A two-wavelength total internal reflection fluorescence method capable of real-time imaging of cellular structure height with nanometre resolution is presented and used to visualize the nanoscale organization of microtubules and endocytosis of the epidermal growth factor receptor.
Journal ArticleDOI
Resolving the spatial relationship between intracellular components by dual color super resolution optical fluctuations imaging (SOFI).
Maria Elena Gallina,Maria Elena Gallina,Jianmin Xu,Thomas Dertinger,Adva Aizer,Yaron Shav-Tal,Shimon Weiss +6 more
TL;DR: This work defines the spatial relationship between hDcp1a, a processing body (P-body, PB) protein, and the tubulin cytoskeletal network and could open up new perspectives on the role of the cytoskeleton in PB formation and assembly.
Posted ContentDOI
DMD-based super-resolution structured illumination microscopy visualizes live cell dynamics at high speed and low cost
TL;DR: Reconstruction of the super-resolved SIM images is achieved on a recently demonstrated parallel-computing platform, which allowed us to visualize living cells with super-resolution at multiple reconstructed frames per second in real time.
Journal ArticleDOI
Enhancing the performance of fluorescence emission difference microscopy using beam modulation
TL;DR: In this paper, the authors theoretically demonstrate that the attainable resolution of FED can be further enhanced by using beam modulation by applying beam shape modulation, polarization state modulating, phase encoding and beam blocking to the illumination beams in the process of illumination beamforming.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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