Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions
TL;DR: New cell-permeable organic fluorescent probes with excellent specificity and high photostability are developed to image the dynamics of lysosomes and their physical interactions with mitochondria using super-resolution microscopy.
Journal ArticleDOI
Tools for the Microbiome: Nano and Beyond.
Julie S. Biteen,Paul C. Blainey,Zoe G. Cardon,Miyoung Chun,George M. Church,Pieter C. Dorrestein,Scott E. Fraser,Jack A. Gilbert,Jack A. Gilbert,Janet K. Jansson,Rob Knight,Jeff F. Miller,Aydogan Ozcan,Kimberly A. Prather,Stephen R. Quake,Edward G. Ruby,Pamela A. Silver,Sharif A. Taha,Ger van den Engh,Paul S. Weiss,Gerard C. L. Wong,Aaron T. Wright,Thomas D. Young +22 more
TL;DR: In this paper, the authors describe the opportunities, technical needs, and potential approaches to address these challenges, based on recent and upcoming advances in measurement and control at the nanoscale and beyond, and provide the basis for advancing the largely descriptive studies of the microbiome to the theoretical and mechanistic understandings that will underpin the discipline of microbiome engineering.
Journal ArticleDOI
Super-resolution fluorescence imaging with single molecules.
Steffen J. Sahl,W. E. Moerner +1 more
TL;DR: This short review summarizes key recent developments and application examples of two-dimensional and three-dimensional multi-color techniques and faster time-lapse schemes and the prospects for quantitative imaging are discussed.
Journal ArticleDOI
SRRF: Universal live-cell super-resolution microscopy.
TL;DR: SRRF can be used to retrieve super-resolution information from most common fluorescence microscopes, and is compatible with any fluorophore, including conventional fluorescent proteins such as GFP.
Journal ArticleDOI
Super-resolution microscopy as a powerful tool to study complex synthetic materials
TL;DR: Super-resolution microscopy (SRM) is emerging as a powerful tool for studying synthetic materials owing to its nanometric resolution, multicolour ability and minimal invasiveness as mentioned in this paper.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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