Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Structured Illumination Microscopy Image Reconstruction Algorithm
Amit Lal,Chunyan Shan,Peng Xi +2 more
TL;DR: The purpose of the present study is to provide an open source SIM reconstruction code (named OpenSIM), which enables users to interactively vary the code parameters and study it's effect on reconstructed SIM image.
Journal ArticleDOI
Single Molecules, Cells, and Super‐Resolution Optics (Nobel Lecture)
TL;DR: In his Nobel Lecture, E. Betzig describes the developments that have led to modern super high-resolution microscopy, and the techniques used to go below the diffraction limit.
Journal ArticleDOI
Lensless digital holographic microscopy and its applications in biomedicine and environmental monitoring
Yichen Wu,Aydogan Ozcan +1 more
TL;DR: The operation principles and the methods behind lensless digital holographic on-chip microscopy are discussed, including some recent work on air quality monitoring, which utilized machine learning for high-throughput and accurate quantification of particulate matter in air.
Journal ArticleDOI
Seeing the forest tree by tree: super-resolution light microscopy meets the neurosciences
TL;DR: New, sometimes surprising answers concerning how protein mobility and protein architectures shape neuronal communication have already emerged and long-term prospects for super-resolution light microscopy in the molecular and cellular neurosciences are concluded.
Journal ArticleDOI
Resolution enhancement techniques in microscopy
TL;DR: A survey of the history of resolution enhancement techniques in microscopy and their impact on current research in biomedicine can be found in this article, where the authors introduce the development of diffraction theory in its relation to enhanced resolution.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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