Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Membrane distribution of the glycine receptor alpha 3 studied by optical super-resolution microscopy
Kristof Notelaers,Kristof Notelaers,Susana Rocha,Rik Paesen,Nina Swinnen,Jeroen Vangindertael,Jochen C. Meier,Jean-Michel Rigo,Marcel Ameloot,Johan Hofkens +9 more
TL;DR: It is demonstrated that RNA splicing determines GlyR α3 membrane distribution, which has consequences for neuronal GlyR physiology and function.
Journal ArticleDOI
Intracellular Ca 2+ release decelerates mitochondrial cristae dynamics within the junctions to the endoplasmic reticulum
Benjamin Gottschalk,Christinae Klec,Markus Waldeck-Weiermair,Roland Malli,Wolfgang F. Graier +4 more
TL;DR: A robust super-resolution method is presented to quantify the dynamics of mitochondrial cristae, the main substructures of the inner mitochondrial membrane, exploiting structured illumination microscopy (SIM), and suggests that local Ca2+ signals specifically control CM-dynamics and structure to facilitate a well-balanced functional interplay between mitochondria and the ER.
Journal ArticleDOI
Wide-field super-resolution optical sectioning microscopy using a single spatial light modulator
TL;DR: In this article, a liquid-crystal spatial light modulator was employed to perform two-dimensional structured light illumination in a conventional wide-field optical microscope, which achieved a depth resolution of 0.29 wavelengths.
Journal ArticleDOI
Heterochromatic Nonlinear Optical Responses in Upconversion Nanoparticles for Super‐Resolution Nanoscopy
TL;DR: In this paper, a point spread function (PSF) engineering by an emitter's response can code higher-spatial-frequency information of an image for microscopy to achieve super-resolution.
Journal ArticleDOI
Podosomes revealed by advanced bioimaging: What did we learn?
TL;DR: This concise review discusses how the applications of fluorescence based techniques that circumvent the diffraction limit of conventional light microscopy took a major leap forward and increased the understanding of the podosome ultra-structure and function.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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