Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Analytical characterisation of nanoscale zero-valent iron: A methodological review
Laura Chekli,B Bayatsarmadi,Ryo Sekine,Binoy Sarkar,AM Shen,Kirk G. Scheckel,William Skinner,Ravi Naidu,Ravi Naidu,Ho Kyong Shon,Ho Kyong Shon,Enzo Lombi,Erica Donner,Erica Donner +13 more
TL;DR: A critical review of their usefulness and limitations for nZVI characterisation is provided and example characterisation data derived from commercial nZ VI materials is used to further illustrate method strengths and limitations.
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Photonic crystal enhanced microscopy for imaging of live cell adhesion
Weili Chen,Kenneth D. Long,Meng Lu,Vikram Chaudhery,Hojeong Yu,Ji Sun Choi,James Polans,Yue Zhuo,Brendan A.C. Harley,Brian T. Cunningham +9 more
TL;DR: PCEM provides rich, dynamic information about the evolution of cell-surface attachment profiles over biologically relevant time-scales and retains the ability to monitor cell behavior with spatial resolution sufficient for observing both attachment footprints of filopodial extensions and intracellular attachment strength gradients.
Journal ArticleDOI
Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes
Matthew A. Nystoriak,Madeline Nieves-Cintrón,Tommaso Patriarchi,Olivia R. Buonarati,Maria Paz Prada,Stefano Morotti,Eleonora Grandi,Julia Dos Santos Fernandes,Katherine A. Forbush,Franz Hofmann,Kent C. Sasse,John D. Scott,Sean M. Ward,Johannes W. Hell,Manuel F. Navedo +14 more
TL;DR: Findings reveal an essential role for α1C phosphorylation at Ser1928 in stimulating CaV1.2 channel activity and vasoconstriction by AKAP-targeted PKA upon exposure to increased glucose and in diabetes.
Journal ArticleDOI
Super-resolution Microscopy in Plant Cell Imaging
TL;DR: The basic principles of existing super-resolution methods are summarized and examples of applications in plant science are provided and the potential for future Applications in plant cell imaging is highlighted.
Journal ArticleDOI
Super-resolution spinning-disk confocal microscopy using optical photon reassignment
Takuya Azuma,Takayuki Kei +1 more
TL;DR: A super-resolution method based on spinning-disk confocal microscopy that optically improves lateral resolution by a factor of 1.37 with a single exposure is reported that will enable reliable observations at super high resolution in biomedical routine research.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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