Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Patent
Process, System and Software Arrangement for Measuring a Mechanical Strain and Elastic Properties of a Sample
TL;DR: In this paper, a system, process and software arrangement are provided to determine data associated with at least one structural change of tissue, and the modulus of the tissue may be determined as a function of the received OCT signal.
Journal ArticleDOI
Advances in tomography: probing the molecular architecture of cells
TL;DR: By combining cryo-ET with super-resolution fluorescence microscopy approaches, it should be possible to localize proteins with high precision inside cells and so elucidate a more realistic view of cellular processes.
Review PALM and STORM: What hides beyond the Rayleigh limit?
TL;DR: The development of photo‐switchable fluorophores, high‐sensitivity microscopes and single particle localization algorithms, and emerging areas where super‐resolution will give fundamental new “eye” sight to cell biology are discussed.
Journal ArticleDOI
Incisive Imaging and Computation for Cellular Mysteries: Lessons from Abscission
TL;DR: Imaging innovations that helped answer long-standing questions about the mechanism of abscission are described and computational modeling of high-resolution data was employed to test hypotheses and generate additional insights.
Journal ArticleDOI
Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging
Chieh-Han Lu,Wei-Chun Tang,Yen-Ting Liu,Shu-Wei Chang,Frances Camille M. Wu,Chin-Yi Chen,Yun-Chi Tsai,Shun-Min Yang,Chiung-Wen Kuo,Yasushi Okada,Yeukuang Hwu,Peilin Chen,Bi-Chang Chen,Bi-Chang Chen +13 more
TL;DR: A fast, large-scale, and three-dimensional super-resolution fluorescence microscope based on single-wavelength Bessel lightsheet to selectively illuminate spontaneous blinking fluorophores tagged to the proteins of interest in space and a spontaneously blinking dye for localization-based imaging is presented.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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