Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
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Journal ArticleDOI
Cadherin-13 Deficiency Increases Dorsal Raphe 5-HT Neuron Density and Prefrontal Cortex Innervation in the Mouse Brain.
Andrea Forero,Olga Rivero,Sina Wäldchen,Hsing-Ping Ku,Dominik P. Kiser,Yvonne Gärtner,Laura S. Pennington,Jonas Waider,Patricia Gaspar,Charline Jansch,Frank Edenhofer,Thérèse J. Resink,Robert Blum,Markus Sauer,Klaus-Peter Lesch,Klaus-Peter Lesch,Klaus-Peter Lesch +16 more
TL;DR: It is indicated that Cdh13 deficiency affects the cell density of the developing DR and the posterior innervation of the prefrontal cortex (PFC), and therefore might be involved in the migration, axonal outgrowth and terminal target finding of DR 5-HT neurons.
Journal ArticleDOI
GMars-Q Enables Long-Term Live-Cell Parallelized Reversible Saturable Optical Fluorescence Transitions Nanoscopy.
TL;DR: It is demonstrated that GMars-Q, a monomeric RSFP with low residual off-state fluorescence and strong fatigue resistance attributed to a biphasic photobleaching process, is particularly suitable for long-term parallelized RESOLFT nanoscopy as it supports an order of magnitude longer imaging durations than existing RSFPs.
Journal ArticleDOI
Determination of dendritic spine morphology by the striatin scaffold protein STRN4 through interaction with the phosphatase PP2A
TL;DR: The local expression of STRN4 is unraveled as a novel mechanism for the control of dendritic spine morphology and the function of various striatin family members in the neuron remains unknown.
Journal ArticleDOI
A matter of scale: how emerging technologies are redefining our view of chromosome architecture.
TL;DR: This review describes how the use of new technologies is starting to revolutionize the field of chromosome organization, and to shed light on the mechanisms of transcription, replication, and repair.
Journal ArticleDOI
Imaging tripartite synapses using super-resolution microscopy
TL;DR: SMLM reveals contiguous scatters of GLT-1 molecules near excitatory synapses in three-colour 3D dSTORM, which is adaptable to various tissues and targets.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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