Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Patent
Systems, devices, methods, apparatus and computer-accessible media for providing optical imaging of structures and compositions
Guillermo J. Tearney,Brett E. Bouma,Hongki Yoo,Milen Shishkov,Joseph A. Gardecki,Roman Shubochkin,Theodore F. Morse,Amin Farouc Jaffer +7 more
TL;DR: In this article, the authors describe systems, devices, methods, apparatus and computer-accessible media for providing and/or utilizing optical frequency domain imaging (OFDI) and fluorescence of structures.
Journal ArticleDOI
Image scanning microscopy.
Ingo Gregor,Jörg Enderlein +1 more
TL;DR: The review gives a brief introduction into the basic principles of ISM, and it reports about recent progress and applications of this new microscopy technique.
Journal ArticleDOI
Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution
Lin Wang,Benji C. Bateman,Laura C. Zanetti-Domingues,Amy N. Moores,Sam Astbury,C. Spindloe,Michele C. Darrow,Maria Romano,Maria Romano,Sarah R. Needham,Konstantinos Beis,Konstantinos Beis,Daniel J. Rolfe,David T. Clarke,Marisa L. Martin-Fernandez +14 more
TL;DR: A new super-resolution modality using a super-hemispherical solid immersion lens is presented, which offers the technical means to study bacterial and mammalian cell samples at molecule localisation length-scales and opens a straightforward route to achieve unmatched resolution on bacterial and mammals cell samples.
Journal ArticleDOI
Structured illumination microscopy of autofluorescent aggregations in human tissue.
Gerrit Best,Roman Amberger,David Baddeley,David Baddeley,Thomas Ach,Stefan Dithmar,Rainer Heintzmann,Christoph Cremer +7 more
TL;DR: This work used Structured Illumination Microscopy with three different excitation wavelengths (488, 568 and 647 nm) to gather spectral information about the autofluorescence signal, thus enabling the collection of light-optical images of autoflorescence distributions in the tissue with previously unmatched optical resolution.
Journal ArticleDOI
Single-molecule super-resolution light-sheet microscopy.
TL;DR: An overview of the recent developments that address the potential of combining light-sheet microscopy and localization-based super-resolution imaging to achieve sub-diffraction-limited resolution is provided.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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