Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation.
TL;DR: In this article, a compressed sensing approach was proposed to obtain 3D image information in 3D from a 2D scan by exploiting orthogonal speckle illumination patterns and compressed sensing of the sparse fluorescence.
Journal ArticleDOI
Conical diffraction illumination opens the way for low phototoxicity super-resolution imaging
Julien Caron,Clément Fallet,Jean-Yves Tinevez,Lionel Moisan,L Philippe Ori Braitbart,Gabriel Y. Sirat,Spencer L. Shorte +6 more
TL;DR: This article presents the first implementation of BSR modality on a commercial confocal microscope, acquiring and analyzing validation data, showing high quality super-resolved images of biological objects, and demonstrating the wide applicability of the technology.
Book ChapterDOI
Structured Illumination Microscopy
TL;DR: An introduction to the principles of SIM is provided, its impact in cell biology is detail, and the most recent developments of this imaging technology are presented.
Posted ContentDOI
Single-cell absolute contact probability detection reveals that chromosomes are organized by multiple, low-frequency yet specific interactions
Diego I. Cattoni,Andrés M. Cardozo Gizzi,Mariya Georgieva,Marco Di Stefano,Alessandro Valeri,Delphine Chamousset,Christophe Houbron,Stéphanie Déjardin,Jean-Bernard Fiche,Inma González,Jia-Ming Chang,Thomas Sexton,Marc A. Marti-Renom,Frédéric Bantignies,Giacomo Cavalli,Marcelo Nollmann +15 more
TL;DR: In this paper, the authors combined high-content super-resolution microscopies with state-of-the-art DNA labeling methods to reveal the variability in the multiscale organization of the Drosophila genome.
Journal ArticleDOI
Quantum limits to optical point-source localization
TL;DR: In this paper, the authors derived quantum lower bounds on the error of locating point sources in free space, taking full account of the quantum, non-paraxial, and vectoral nature of photons.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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