Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Rapid quantum image scanning microscopy by joint sparse reconstruction
TL;DR: Enhanced superresolved reconstructions from short scans of a biological sample labeled with quantum dots are obtained, demonstrating the potential of the quantum image scanning microscopy method for quantum imaging in life science microscopy.
Journal ArticleDOI
Structured illumination in compact and field-portable 3D-printed shearing digital holographic microscopy for resolution enhancement.
TL;DR: This is the first report of a compact and field-portable SI digital holographic system based on shearing geometry and the experimental results for the USAF resolution target show a resolution improvement of a factor of two which corroborates the theoretical prediction.
Journal ArticleDOI
Fast TIRF-SIM imaging of dynamic, low-fluorescent biological samples
TL;DR: A TIRF-SIM system based on scan-mirrors and a Michelson interferometer, which generates images at 110 nm spatial resolution and up to 8 Hz temporal resolution, and a framework and guidelines on how the modulation contrast, which depends on laser coherence, polarization, beam displacement or sample movements, can be mapped over the entire field of view.
Journal ArticleDOI
Expansion microscopy: A powerful nanoscale imaging tool for neuroscientists.
Brendan Gallagher,Yongxin Zhao +1 more
TL;DR: ExpMicroscopy (ExM) as discussed by the authors is an alternative solution to overcome the diffraction limit by physically magnifying biological specimens, including nervous systems, and their applications to synaptic imaging, neuronal tracing, and the study of neurological disease.
Journal ArticleDOI
Super-resolution fluorescence polarization microscopy
Karl Zhanghao,Karl Zhanghao,Juntao Gao,Dayong Jin,Xuedian Zhang,Xuedian Zhang,Peng Xi,Peng Xi +7 more
TL;DR: In this review, both diffraction limited and super resolution fluorescence polarization microscopy techniques, as well as their applications in biological imaging are summarized.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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