Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Metamaterials and imaging.
Minkyung Kim,Junsuk Rho +1 more
TL;DR: In this paper, a review of metamaterial-based lenses which offer the new types of imaging components and functions is presented. But not all of them offer sub-diffraction imaging, but they provide new imaging mechanisms by controlling and manipulating the path of light.
Journal ArticleDOI
Flexible structured illumination microscope with a programmable illumination array.
TL;DR: This work developed a unique and highly accurate calibration approach which allowed it to determine a mathematical model describing the mapping of the illumination pattern from the microdisplay to the camera sensor, important for higher performance image processing methods such as scaled subtraction of the out of focus light.
Journal ArticleDOI
Polarization sensitive, three-dimensional, single-molecule imaging of cells with a double-helix system
TL;DR: Information obtained from the two polarization channels demonstrates polarization based contrast in 3D superresolution imaging and is optimally combined to yield up to 30% improvement in localization precision relative to a single polarization channel system.
Journal ArticleDOI
VASP, zyxin and TES are tension-dependent members of Focal Adherens Junctions independent of the α-catenin-vinculin module
Joppe Oldenburg,Gerard van der Krogt,Floor Twiss,Annika Bongaarts,Yasmin Habani,Johan A. Slotman,Adriaan B. Houtsmuller,Stephan Huveneers,Johan de Rooij +8 more
TL;DR: It is concluded that there are multiple force sensitive modules present at the FAJ that are activated at distinct locations along the cadherin-F-actin axis and regulate specific aspects of junction dynamics.
Journal ArticleDOI
Seeing is believing - multi-scale spatio-temporal imaging towards in vivo cell biology.
TL;DR: A palette of imaging methods that are available to the scientific community for elucidating a wide array of biological events is reviewed, covering the most-recent developments in intravital imaging, light-sheet microscopy, super-resolution imaging, and correlative light and electron microscopy.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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