Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Overexpression of fetA (ybbL) and fetB (ybbM), Encoding an Iron Exporter, Enhances Resistance to Oxidative Stress in Escherichia coli
TL;DR: Findings show that fetA and fetB encode an iron exporter that has a role in enhancing resistance to H2O2-mediated oxidative stress and can minimize oxidative stress under conditions of iron overload and suggest that FetAB facilitates iron homeostasis to decrease oxidative stress.
Journal ArticleDOI
Complex vectorial optics through gradient index lens cascades
Chao He,Jintao Chang,Qi Hu,Jingyu Wang,Jacopo Antonello,Honghui He,Shaoxiong Liu,Jianyu Lin,Ben Dai,Daniel S. Elson,Peng Xi,Hui Ma,Martin J. Booth +12 more
TL;DR: In this paper, the authors harnessed the birefringence of GRIN lenses in cascade with other optical components to enable extra functionality in commonplace GRIN lens systems, such as vector vortex beams and foci.
Journal ArticleDOI
Real-time visualization of the cytoskeleton and effector functions in T cells
TL;DR: This work focuses on the use of fluorescent fusion proteins combined with high resolution imaging techniques, including confocal and multiphoton microscopy, for the visualization of cytoskeleton-dependent processes in living cells.
Journal ArticleDOI
Image scanning microscopy with a quadrant detector.
TL;DR: Here it is shown that ISM can be straightforwardly implemented by substituting the single point detector of a confocal microscope with a quadrant detector of the same size, thus using a small number of detector elements.
Journal ArticleDOI
Localization Microscopy using Noncovalent Fluorogen Activation by Genetically Encoded Fluorogen‐Activating Proteins.
Qi Yan,Samantha L. Schwartz,Suvrajit Maji,Suvrajit Maji,Fang Huang,Chris Szent-Gyorgyi,Diane S. Lidke,Keith A. Lidke,Marcel P. Bruchez +8 more
TL;DR: The super-resolution properties and images obtained from a selected FAP clone fused to actin are evaluated, and it is shown that the photon counts per object are between those typically reported for fluorescent proteins and switching-dye pairs, resulting in 10-30 nm localization precision per object.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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