Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Speed limits of structured illumination microscopy
TL;DR: The results show that there exists a limit above which deconvolution microscopy becomes superior to SIM and the effect of the sample movement on the reconstruction quality and the number of raw frames recordable.
Journal ArticleDOI
Shedding new light on viruses:: super-resolution microscopy for studying human immunodeficiency virus
TL;DR: Findings on human immunodeficiency virus (HIV-1) obtained using SR-FM techniques are summarized and indicate the potential to approach open questions in HIV-1 replication from a new angle.
Journal ArticleDOI
Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics"
Steffen J. Sahl,Francisco Balzarotti,Jan Keller-Findeisen,Marcel Leutenegger,Volker Westphal,Alexander Egner,Flavie Lavoie-Cardinal,Andriy Chmyrov,Tim Grotjohann,Stefan Jakobs,Stefan Jakobs +10 more
TL;DR: This work questions the methods’ reliability to visualize specimen features at sub–100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features.
Journal ArticleDOI
Phase retrieval with resolution enhancement by using structured illumination.
TL;DR: This Letter presents referenceless phase retrieval methods with resolution enhancement and generates diffraction patterns that are reconstructed from the phase of the wavefront from Structured illuminations generated by a spatial light modulator.
Journal ArticleDOI
High speed structured illumination microscopy in optically thick samples.
TL;DR: By combining a single plane reconstruction algorithm with hardware for high-speed switching between illumination patterns and rapid acquisition of fluorescence images, this paper demonstrates high speed super-resolution imaging inside biological organisms.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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