Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TLDR
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.Abstract:
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high-resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.read more
Citations
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Journal ArticleDOI
Rapid staining and imaging of subnuclear features to differentiate between malignant and benign breast tissues at a point-of-care setting
Jenna L. Mueller,Jennifer Gallagher,Rhea Chitalia,Marlee S. Krieger,Alaattin Erkanli,Rebecca Willett,Joseph Geradts,Nimmi Ramanujam +7 more
TL;DR: Results indicate that the quantitative microscopy toolbox is a potentially viable approach for detecting the presence of malignancy in clinical core needle breast biopsies and develop a strategy to segment and quantify breast tissue features in order to enable automated tissue diagnosis.
Journal ArticleDOI
Computational coherent imaging by rotating a cylindrical lens
TL;DR: To overcome longitudinal sampling rate fluctuation in axial multi-image computational imaging, an effortless and high-efficient optical scanning imaging system via the rotation of single cylindrical lens (RSCL) is proposed for reconstructing the amplitude and phase information of sample.
Journal ArticleDOI
Structured illumination microscopy with interleaved reconstruction (SIMILR).
TL;DR: A new SR image reconstruction method that maximizes the use of each subframe of the acquisition series is proposed for improving the super‐resolved frame rate by N times for N illumination directions.
Book ChapterDOI
3D subcellular localization with superresolution array tomography on ultrathin sections of various species
Sebastian M. Markert,Vivien Bauer,Thomas S. Muenz,Nicola G. Jones,Frederik Helmprobst,Sebastian Britz,Markus Sauer,Wolfgang Rössler,Markus Engstler,Christian Stigloher +9 more
TL;DR: A step-by-step recipe for superresolution AT that can be easily applied for C. elegans, T. brucei, C. fortis, and A. mellifera is offered and adapted for other model systems.
Journal ArticleDOI
Experimental Demonstration of Hyperbolic Metamaterial Assisted Illumination Nanoscopy
Qian Ma,Haoliang Qian,S. A. Montoya,Wei Bao,Lorenzo Ferrari,Huan Hu,Emroz Khan,Yuan Wang,Eric E. Fullerton,Evgenii E. Narimanov,Xiang Zhang,Zhaowei Liu +11 more
TL;DR: This paper experimentally demonstrates the concept of hyperstructured illumination and achieves a ∼80 nm resolution in a well-known Ag/SiO2 multilayer HMM system by using a low numerical aperture objective, representing a 6-fold resolution enhancement of the diffraction limit.
References
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BookDOI
Handbook of biological confocal microscopy
TL;DR: Methods for Three-Dimensional Imaging and Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen.
Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TL;DR: A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.
Journal ArticleDOI
Subdiffraction resolution in far-field fluorescence microscopy.
Thomas A. Klar,Stefan W. Hell +1 more
TL;DR: The resolution limit of scanning far-field fluorescence microscopy is overcame by disabling the fluorescence from the outer part of the focal spot by a spatially offset pulse.
Book ChapterDOI
Fluorescence microscopy in three dimensions.
TL;DR: This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen.
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