Steven P. Gygi

TL;DR: In this article, the authors demonstrate that the actin cytoskeleton polarization of yeast inhibits the highly conserved Target of Rapamycin Complex 1 (TORC1) pathway, and that this mechanism serves to coordinate the ability of cells to increase in size with their biosynthetic capacity.

TL;DR: Surprisingly, Pup and PafA were sufficient to pupylate at least 51 E. coli proteins in addition to the mycobacterial proteins, suggesting that pupylation signals are intrinsic to targeted proteins and might not require Mycobacterium‐specific cofactors for substrate recognition by PAFA in vivo.

TL;DR: It is shown that using stable isotope labeling with amino acids, mass spectrometry based quantitative proteomics could detect 26 of the 33 50S and 20 of the 21 30S subunit r-proteins when coexpressed in batch format PURE system and points to what is still needed to obtain self-replicating synthetic ribosomes in situ in the PURES system.

TL;DR: Perturbations in protein levels resulting from nicotine treatment in four cell lines are measured using tandem mass tags (TMT10‐plex) and high‐resolution mass spectrometry to highlight proteins, including APP, APLP2, LAPTM4B, and NCOA4, which were dysregulated by nicotine in all cell lines investigated and may have implications in downstream signaling pathways, particularly autophagy.

TL;DR: This work presents Combinatorial Isobaric Mass Tags (CMTs), an isobaric labeling architecture with the unique ability to generate multiple series of reporter ions simultaneously, and demonstrates that utilization of multiple reporter ion series improves multiplexing capacity of CMT.