S
Steven P. Gygi
Researcher at Harvard University
Publications - 778
Citations - 147003
Steven P. Gygi is an academic researcher from Harvard University. The author has contributed to research in topics: Proteome & Phosphorylation. The author has an hindex of 172, co-authored 704 publications receiving 129173 citations. Previous affiliations of Steven P. Gygi include University of Rochester Medical Center & Cell Signaling Technology.
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Journal ArticleDOI
Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology
TL;DR: The large range of protein expression levels limits the ability of the 2DE-MS approach to analyze proteins of medium to low abundance, and thus the potential of this technique for proteome analysis is likewise limited.
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RNAi-Mediated Targeting of Heterochromatin by the RITS Complex
André Verdel,Songtao Jia,Scott A. Gerber,Tomoyasu Sugiyama,Steven P. Gygi,Shiv I. S. Grewal,Danesh Moazed +6 more
TL;DR: The purification of an RNAi effector complex termed RITS (RNA-induced initiation of transcriptional gene silencing) that is required for heterochromatin assembly in fission yeast is described and a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochROMatin complexes and epigenetic genesilencing at specific chromosomal loci is suggested.
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Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism
Anne-Claude Gingras,Steven P. Gygi,Brian Raught,Roberto D. Polakiewicz,Robert T. Abraham,Merl F. Hoekstra,Ruedi Aebersold,Nahum Sonenberg +7 more
TL;DR: 4E-BP1 phosphorylation by FRAP/mTOR on Thr-37 and Thr-46 is a priming event for subsequent phosphorylated of the carboxy-terminal serum-sensitive sites, including those that interact with eIF4E.
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The BioPlex Network: A Systematic Exploration of the Human Interactome
Edward L. Huttlin,Lily Ting,Raphael J. Bruckner,Fana Gebreab,Melanie P. Gygi,John Szpyt,Stanley Tam,Gabriela Zarraga,Greg Colby,Kurt Baltier,Rui Dong,Virginia Guarani,Laura Pontano Vaites,Alban Ordureau,Ramin Rad,Brian K. Erickson,Martin Wühr,Joel M. Chick,Bo Zhai,Deepak Kolippakkam,Julian Mintseris,Robert A. Obar,Robert A. Obar,Tim Harris,Spyros Artavanis-Tsakonas,Spyros Artavanis-Tsakonas,Mathew E. Sowa,Pietro De Camilli,Joao A. Paulo,J. Wade Harper,Steven P. Gygi +30 more
TL;DR: Using high-throughput affinity-purification mass spectrometry, BioPlex is used to identify interacting partners for 2,594 human proteins in HEK293T cells and reveals associations among thousands of protein domains, suggesting a basis for examining structurally related proteins.
Journal ArticleDOI
Architecture of the human interactome defines protein communities and disease networks
Edward L. Huttlin,Raphael J. Bruckner,Joao A. Paulo,Joe R. Cannon,Lily Ting,Kurt Baltier,Greg Colby,Fana Gebreab,Melanie P. Gygi,Hannah Parzen,John Szpyt,Stanley Tam,Gabriela Zarraga,Laura Pontano-Vaites,Sharan Swarup,Anne E. White,Devin K. Schweppe,Ramin Rad,Brian K. Erickson,Robert A. Obar,Robert A. Obar,K. G. Guruharsha,Kejie Li,Spyros Artavanis-Tsakonas,Spyros Artavanis-Tsakonas,Steven P. Gygi,J. Wade Harper +26 more
TL;DR: With more than 56,000 candidate interactions, BioPlex 2.0 exceeds previous experimentally derived interaction networks in depth and breadth, and will be a valuable resource for exploring the biology of incompletely characterized proteins and for elucidating larger-scale patterns of proteome organization.