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Steven P. Gygi
Researcher at Harvard University
Publications - 778
Citations - 147003
Steven P. Gygi is an academic researcher from Harvard University. The author has contributed to research in topics: Proteome & Phosphorylation. The author has an hindex of 172, co-authored 704 publications receiving 129173 citations. Previous affiliations of Steven P. Gygi include University of Rochester Medical Center & Cell Signaling Technology.
Papers
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Journal ArticleDOI
Time-resolved proximity labeling of protein networks associated with ligand-activated EGFR
Mireia Perez Verdaguer,Tian Zhang,Sachin V. Surve,Joao A. Paulo,Callen T. Wallace,Simon C. Watkins,Steven P. Gygi,Alexander Sorkin +7 more
TL;DR: In this paper , peroxidase-catalyzed proximity labeling, isobaric peptide tagging, and quantitative mass spectrometry were combined to define the dynamics of the proximity proteome of ligand-activated EGFR.
Journal ArticleDOI
Adiporedoxin, an upstream regulator of ER oxidative folding and protein secretion in adipocytes
Mark P. Jedrychowski,Mark P. Jedrychowski,Libin Liu,Collette J. Laflamme,Kalypso Karastergiou,Tova Meshulam,Shi-Ying Ding,Yuanyuan Wu,Mi-Jeong Lee,Steven P. Gygi,Susan K. Fried,Paul F. Pilch +11 more
TL;DR: Data support the notion that Adrx plays a critical role in adipocyte biology and in the regulation of mouse and human metabolism via its modulation of adipocyte protein secretion.
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Apolipoprotein E is a pancreatic extracellular factor that maintains mature β-cell gene expression.
Ahmed I. Mahmoud,Francisco X Galdos,Katherine A Dinan,Mark P. Jedrychowski,Jeffrey C. Davis,Ana Vujic,Inbal Rachmin,Christian Shigley,James R. Pancoast,Samuel C. K. Lee,Jennifer Hollister-Lock,Catherine MacGillivray,Steven P. Gygi,Douglas A. Melton,Gordon C. Weir,Richard T. Lee +15 more
TL;DR: It is shown that after isolation and during maintenance in culture, adherent rat islets reduce expression of key β-cell transcription factors necessary forβ-cell function and that soluble pancreatic decellularized matrix (DCM) can enhance β- cell gene expression.
Posted ContentDOI
Time resolved quantitative phosphoproteomics reveals distinct patterns of SHP2 dependence in EGFR signaling
Vemulapalli,Lily A. Chylek,Alison R. Erickson,Jonathan R. LaRochelle,Kartik Subramanian,Movarid Mohseni,Matthew J. LaMarche,Mike Acker,Peter K. Sorger,Steven P. Gygi,Stephen C. Blacklow +10 more
TL;DR: Monitoring phosphoproteome dynamics when SHP2 is allosterically inhibited by the small molecule SHP099 reveals six distinct response signatures following SHp099 treatment and washout, highlighting how analysis of phospho-abundance dynamics can provide insight into transmembrane signaling responses.
Journal ArticleDOI
Fe3+-NTA magnetic beads as an alternative to spin column-based phosphopeptide enrichment.
Xinyue Liu,Valentina Rossio,Sanjukta Guha Thakurta,Amarjeet Flora,Leigh Foster,Ryan Bomgarden,Steven P. Gygi,Joao A. Paulo +7 more
TL;DR: In this article , a spin column-based Fe3+-NTA enrichment strategy was compared with one encompassing magnetic beads for global protein phosphorylation, and the results showed that the scalable and automation-friendly magnetic bead-based workflow is an equivalent, but more flexible, enrichment strategy for phosphoproteome profiling experiments.