S
Steven P. Gygi
Researcher at Harvard University
Publications - 778
Citations - 147003
Steven P. Gygi is an academic researcher from Harvard University. The author has contributed to research in topics: Proteome & Phosphorylation. The author has an hindex of 172, co-authored 704 publications receiving 129173 citations. Previous affiliations of Steven P. Gygi include University of Rochester Medical Center & Cell Signaling Technology.
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Journal ArticleDOI
Structural Defects in the Regulatory Particle-Core Particle Interface of the Proteasome Induce a Novel Proteasome Stress Response
TL;DR: Proteasome remodeling through the addition of Ecm29 and Hul5 suggests a new layer of the proteasome stress response and may be a common response to structurally aberrant proteasomes or deficient prote asome function.
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Phosphorylation of ETS1 by Src family kinases prevents its recognition by the COP1 tumor suppressor.
Gang Lu,Qing Zhang,Ying Huang,Jiaxi Song,Ross Tomaino,Tobias Ehrenberger,Elgene Lim,Wenbin Liu,Roderick T. Bronson,Michaela Bowden,Jane E. Brock,Ian E. Krop,Deborah A. Dillon,Steven P. Gygi,Gordon B. Mills,Andrea L. Richardson,Sabina Signoretti,Michael B. Yaffe,William G. Kaelin,William G. Kaelin +19 more
TL;DR: Findings expand the list of potential COP1 substrates to include proteins whose COP1-binding sites are subject to regulatory phosphorylation and provide insights into transformation by Src family kinases.
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Effects of MEK inhibitors GSK1120212 and PD0325901 in vivo using 10‐plex quantitative proteomics and phosphoproteomics
Joao A. Paulo,Fiona E. McAllister,Robert A. Everley,Sean A. Beausoleil,Alexander S. Banks,Steven P. Gygi +5 more
TL;DR: Phosphorylation motif analysis revealed that the MEK inhibitors decreased phosphorylation levels of proline‐x‐serine‐proline (PxSP) and serine‐ Proline (SP) sites, consistent with extracellular‐signal‐regulated kinase (ERK) inhibition.
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Increasing throughput in targeted proteomics assays: 54-plex quantitation in a single mass spectrometry run.
TL;DR: The 54-plex approach resulted in a significant reduction in purification resources (time, reagents, etc.) and a ~50-fold improvement in acquisition throughput and was demonstrated in several ways including measuring inhibition of PKA activity in MCF7 cell lysates for a panel of nine compounds.
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Reduced MEK inhibition preserves genomic stability in naive human embryonic stem cells
Bruno Di Stefano,Mai Ueda,Shan Sabri,Justin Brumbaugh,Aaron J. Huebner,Anna Sahakyan,Kendell Clement,Kendell Clement,Katie J. Clowers,Alison R. Erickson,Keiko Shioda,Steven P. Gygi,Hongcang Gu,Hongcang Gu,Toshi Shioda,Alexander Meissner,Alexander Meissner,Yasuhiro Takashima,Kathrin Plath,Konrad Hochedlinger +19 more
TL;DR: Modifications to standard culture conditions are described that permit the growth of naive human pluripotent stem cells with reduced genomic instability and provide a simple modification to current methods that can enable robust growth and reduced genomic stability in naive hESCs.