S
Steven P. Gygi
Researcher at Harvard University
Publications - 778
Citations - 147003
Steven P. Gygi is an academic researcher from Harvard University. The author has contributed to research in topics: Proteome & Phosphorylation. The author has an hindex of 172, co-authored 704 publications receiving 129173 citations. Previous affiliations of Steven P. Gygi include University of Rochester Medical Center & Cell Signaling Technology.
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Journal ArticleDOI
During muscle atrophy, thick, but not thin, filament components are degraded by MuRF1-dependent ubiquitylation
Shenhav Cohen,Jeffrey J. Brault,Steven P. Gygi,David J. Glass,David M. Valenzuela,Carlos A. Gartner,Esther Latres,Alfred L. Goldberg +7 more
TL;DR: This work generated mice expressing a Ring-deletion mutant MuRF1, which binds but cannot ubiquitylate substrates, and identified many myofibrillar components in denervated muscle that were decreased by a mechanism not requiring Mu RF1.
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Regulation of monoubiquitinated PCNA by DUB autocleavage
Tony T. Huang,Sebastian M.B. Nijman,Kanchan D. Mirchandani,Paul J. Galardy,Martin A. Cohn,Wilhelm Haas,Steven P. Gygi,Hidde L. Ploegh,René Bernards,Alan D. D'Andrea +9 more
TL;DR: This work shows that the DUB ubiquitin specific protease 1 (USP1) deubiquitinates the DNA replication processivity factor, PCNA, as a safeguard against error-prone translesion synthesis (TLS) of DNA.
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Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses.
Graeme C. McAlister,Edward L. Huttlin,Wilhelm Haas,Lily Ting,Mark P. Jedrychowski,John C. Rogers,Karsten Kuhn,Ian Pike,Robert A. Grothe,Justin D. Blethrow,Steven P. Gygi +10 more
TL;DR: This work serves as a blueprint for expanding the multiplexing capacity of the TMT reagents to at least 10-plex and possibly up to 18-plex, and is able to quantify across eight samples simultaneously by combining the (13)C- and (15)N-containing reporter ions.
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A chromatin localization screen reveals poly (ADP ribose)-regulated recruitment of the repressive polycomb and NuRD complexes to sites of DNA damage
Danny M. Chou,Brittany Susan Adamson,Noah Dephoure,Xu Tan,Amanda C. Nottke,Kristen E. Hurov,Steven P. Gygi,Monica P. Colaiácovo,Stephen J. Elledge +8 more
TL;DR: It is proposed that PARP sets up a transient repressive chromatin structure at sites of DNA damage to block transcription and facilitate DNA repair.
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Quantitative Proteomics of the Cancer Cell Line Encyclopedia
David P. Nusinow,John Szpyt,Mahmoud Ghandi,Christopher M. Rose,E. Robert McDonald,Marian Kalocsay,Judit Jané-Valbuena,Ellen Gelfand,Devin K. Schweppe,Mark P. Jedrychowski,Javad Golji,Dale Porter,Tomas Rejtar,Y. Karen Wang,Gregory V. Kryukov,Frank Stegmeier,Brian K. Erickson,Levi A. Garraway,Levi A. Garraway,William R. Sellers,Steven P. Gygi +20 more
TL;DR: An analysis of microsatellite instable (MSI) cell lines reveals the dysregulation of specific protein complexes associated with surveillance of mutation and translation and these and other protein complexes were associated with sensitivity to knockdown of several different genes.