Steven P. Gygi

TL;DR: This work identifies numerous stereoselective protein–fragment interactions in cells and shows that these interactions occur at functional sites on proteins from diverse classes, indicating that incorporating chirality into fully functionalized fragment libraries provides a robust and streamlined method to discover ligandable proteins in cells.

TL;DR: The findings demonstrate a link between oncogene and growth factor signaling and chaperonin CCT-mediated cellular activities and show that RNA interference-mediated knockdown of endogenous CCTβ causes impaired cell proliferation that can be rescued with ectopically expressed murine CCT β wild-type or phosphomimetic mutant S260D, but not the phosphorylation-deficient mutant S 260A.

TL;DR: A model is provided in which WDR20 serves as a stimulatory subunit for preserving and regulating the activity of the subset of the UAF1·USP complexes, which contradicts the previous report that USP12 and USP46 do not regulate the FA pathway.

TL;DR: It is shown that addition of proline-arginine (PR) and glycine-argarine (GR) toxic DPR peptides to nuclear extracts blocks spliceosome assembly and splicing, but not other types of RNA processing.

TL;DR: In this paper, the authors performed temporal quantitative proteomics to fully capture protein induction and repression during the diauxic shift in yeast and achieved accurate and sensitive quantitation at a high temporal resolution and depth of proteome coverage was achieved using TMT10 reagents and LC-MS3 analysis on an Orbitrap Fusion tribrid mass spectrometer deploying synchronous precursor selection.