Ladoke Akintola University of Technology

About: Ladoke Akintola University of Technology is a education organization based out in Ogbomoso, Nigeria. It is known for research contribution in the topics: Population & Adsorption. The organization has 2786 authors who have published 3066 publications receiving 36850 citations. The organization is also known as: Oyo State University of Technology & LAUTECH.

Instrumental Characterization and Antibacterial Investigation of Silver Nanoparticles Synthesized From Garcinia Kola Leaf

Abstract: The need to devise another method of synthesizing nanoparticles from sources that are eco-friendly, non-hazardous and cost effectiveness is of great importance in preventing environmental and health problems. The aim of this study was to evaluate the efficiency of Garcinia kola leaves as reducing and stabilizing agent for silver nanoparticles synthesis. The leaves of Garcinia kola obtained were authenticated, air dried, pulverized and extracted. The extract was mixed with aqueous solution of silver nitrate solution to form silver nanoparticles and were characterized using Ultra violet (UV) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The antibacterial investigation of the synthesized silver nanoparticle was carried out following the disk diffusion method. UV analysis revealed the silver surface plasmon band at 425.18 nm, The FTIR indicated -OH, -C=C- and alkane as the functional groups responsible for the stabilization of the silver nanoparticle formed. The morphological assessment from SEM and TEM analysis confirmed that the silver nanoparticle formed are spherical in shape with an average particle size of 28.80nm.The EDX analysis ascertained that the silver surface plasmon resonance at 2.8–3.2 keV was confirmed the reduction of silver ion (Ag+ to Ag0). The XRD study revealed the crystalline nature of the nanoparticles synthesized. The antibacterial investigation showed high inhibition against the growth of tested bacteria. This study ascertained that the green synthesis of silver nanoparticle without the use of harmful solvent that are offensive to the environment is achievable. Keywords: Silver nanoparticles, Biosynthesis, Characterization, Antibacterial activity and Garcinia kola.

Rhizopus stolonifer LAU 07: a novel source of fructosyltransferase

Abstract: Increasing awareness of the importance of fructooligosaccharides (FOS) as ingredients of functional foods has led to intensive search of new sources of fructosyltransferases (FTase), enzymes responsible for the conversion of sucrose to fructooligosaccharides. A local strain of Rhizopus stolonifer isolated from spoilt orange fruit with high fructosyltransferase activity (Ut) of 12.31–45.70 U mL−1 during a fermentation period of 24–120 h is herein reported. It showed low hydrolytic activity (Uh) in the range of 0.86–1.78 U mL−1 during the same period. FOS yield of 34 % (1-kestose, GF2, nystose, GF3) was produced by FTase obtained from a 72 h-old culture using 60 g of sucrose per 100 mL of the substrate. When the isolate was grown in a defined submerged medium, its pH dropped sharply from the intial value of 5.5 to 1.0 within 24 h, and this value was maintained throughout the fermentation. The biomass content ranged from 8.8 g L−1 at 24 h of fermentation to reach the maximum of 10 g L−1 at 72 h. It was reduced to 5.6 g L−1 at the end of 120 h of fermentation. This report represents the first reference to a strain of Rhizopus as a source of FTase for the production of FOS. The high Ut/Uh ratio shown by this isolate indicates that it may be a good strain for the industrial and commercial production of FOS. However, there is a need of further optimization of the bioprocess to increase the conversion efficiency of sucrose to FOS by the enzyme.

Occult Hepatitis B Virus Infection among HIV Positive Patients in Nigeria.

Abstract: HIV has been known to interfere with the natural history of hepatitis B virus (HBV) infection. In this study we investigate the prevalence of occult hepatitis B virus infection (OBI) among HIV-infected individuals in Nigeria. Overall, 1200 archived HIV positive samples were screened for detectable HBsAg using rapid technique, in Ikole Ekiti Specialist Hospital. The HBsAg negative samples were tested for HBsAg, anti-HBc, and anti-HCV by ELISA. Polymerase chain reaction was used for HBV DNA amplification and CD4 counts were analyzed by cytometry. Nine hundred and eighty of the HIV samples were HBsAg negative. HBV DNA was detected in 21/188 (11.2%) of patients without detectable HBsAg. CD4 count for the patients ranged from 2 to 2,140 cells/μL of blood (mean = 490 cells/μL of blood). HCV coinfection was detected only in 3/188 (1.6%) of the HIV-infected patients (P > 0.05). Twenty-eight (29.2%) of the 96 HIV samples screened were positive for anti-HBc. Averagely the HBV viral load was <50 copies/mL in the OBI samples examined by quantitative PCR. The prevalence of OBI was significantly high among HIV-infected patients. These findings highlight the significance of nucleic acid testing in HBV diagnosis in HIV patients.

Non-surgical management of congenital eversion of the eyelids.

Abstract: Purpose: To report the effectiveness of non-invasive management of congenital eversion of the eyelids, a rare condition associated with serious socio-psychological consequences. Case Report: Three neonates with congenital eversion of the eyelids and secondary conjunctival chemosis and prolapse were managed with 5% hypertonic normal saline, lubricants, antibiotics, and padding. Complete eye opening was achieved by the 10th day of presentation and the condition resolved. Conclusion: Non-invasive management of congenital eyelid eversion was found to be effective with no need for surgical management. All health care workers should be informed that this condition is amenable to conservative treatment if started early, so that prompt referral for expert management can be offered.