Brains, Genes, and Primates
Juan Carlos Izpisua Belmonte,Edward M. Callaway,Sarah J. Caddick,Patricia Smith Churchland,Guoping Feng,Guoping Feng,Gregg E. Homanics,Kuo-Fen Lee,David A. Leopold,Cory T. Miller,Jude F. Mitchell,Shoukhrat Mitalipov,Shoukhrat Mitalipov,Alysson R. Moutri,J. Anthony Movshon,Hideyuki Okano,Hideyuki Okano,John V. Reynolds,Dario L. Ringach,Terrence J. Sejnowski,Terrence J. Sejnowski,Afonso C. Silva,Peter L. Strick,Peter L. Strick,Jun Wu,Feng Zhang +25 more
TLDR
Several recent advances have been described and their potential to advance the understanding of the human brain and brain disorders, discuss bioethical considerations, and describe what will be needed to move forward are described.About:
This article is published in Neuron.The article was published on 2015-05-06 and is currently open access. It has received 221 citations till now.read more
Citations
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Journal ArticleDOI
In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration
Keiichiro Suzuki,Yuji Tsunekawa,Reyna Hernández-Benítez,Reyna Hernández-Benítez,Jun Wu,Jun Wu,Jie Zhu,Jie Zhu,Euiseok J. Kim,Fumiyuki Hatanaka,Mako Yamamoto,Toshikazu Araoka,Toshikazu Araoka,Zhe Li,Masakazu Kurita,Tomoaki Hishida,Mo Li,Emi Aizawa,Shicheng Guo,Song Chen,April Goebl,Rupa Devi Soligalla,Jing Qu,Tingshuai Jiang,Xin Fu,Xin Fu,Maryam Jafari,Concepcion Rodriguez Esteban,W. Travis Berggren,Jeronimo Lajara,Estrella Núñez-Delicado,Pedro Guillen,Josep M. Campistol,Fumio Matsuzaki,Guang-Hui Liu,Pierre J. Magistretti,Kun Zhang,Edward M. Callaway,Kang Zhang,Juan Carlos Izpisua Belmonte +39 more
TL;DR: The HITI method presented here establishes new avenues for basic research and targeted gene therapies and demonstrates the efficacy of HITI in improving visual function using a rat model of the retinal degeneration condition retinitis pigmentosa.
Journal ArticleDOI
Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain
Benjamin E. Deverman,Piers L. Pravdo,Bryan P. Simpson,Sripriya Ravindra Kumar,Ken Y. Chan,Abhik Banerjee,Wei Li Wu,Bin Yang,Nina Huber,Sergiu P. Paşca,Viviana Gradinaru +10 more
TL;DR: This work uses Cre recombination–based AAV targeted evolution (CREATE) to generate AAV variants that efficiently and widely transduce the adult mouse central nervous system (CNS) after intravenous injection and demonstrates the potential of CREATE to produce customized AAV vectors for biomedical applications.
Journal ArticleDOI
Ischemic stroke: experimental models and reality
TL;DR: The two models mimicking human stroke most closely are various embolic stroke models and spontaneous stroke models, which more closely mimics the therapeutic situation of mechanical thrombectomy which is expected to be increasingly applied to stroke patients.
Journal ArticleDOI
A comprehensive transcriptional map of primate brain development
Trygve E. Bakken,Jeremy A. Miller,Songlin Ding,Susan M. Sunkin,Kimberly A. Smith,Lydia Ng,Aaron Szafer,Rachel A. Dalley,Joshua J. Royall,Tracy Lemon,Sheila Shapouri,Kaylynn Aiona,James M. Arnold,Jeffrey L. Bennett,Darren Bertagnolli,Kristopher Bickley,Andrew F. Boe,Krissy Brouner,Stephanie Butler,Emi J. Byrnes,Shiella Caldejon,Anita Carey,Shelby Cate,Mike Chapin,Jefferey Chen,Nick Dee,Tsega Desta,Tim A. Dolbeare,Nadia Dotson,Amanda Ebbert,Erich Fulfs,Garrett Gee,Terri L. Gilbert,Jeff Goldy,Lindsey Gourley,Ben W. Gregor,Guangyu Gu,Jon Hall,Zeb Haradon,David R. Haynor,Nika Hejazinia,Anna Hoerder-Suabedissen,Robert Howard,Jay Jochim,Marty Kinnunen,Ali Kriedberg,Chihchau L. Kuan,Christopher Lau,Changkyu Lee,Felix Lee,Lon T. Luong,Naveed Mastan,Ryan May,Jose Melchor,Nerick Mosqueda,Erika Mott,Kiet Ngo,Julie Nyhus,Aaron Oldre,Eric Olson,Jody Parente,Patrick D. Parker,Sheana Parry,Julie Pendergraft,Lydia Potekhina,Melissa Reding,Zackery L. Riley,Tyson Roberts,Brandon Rogers,Kate Roll,David Rosen,David Sandman,Melaine Sarreal,Nadiya V. Shapovalova,Shu Shi,Nathan Sjoquist,Andy J. Sodt,Robbie Townsend,Lissette Velasquez,Udi Wagley,Wayne Wakeman,Cassandra White,Crissa Bennett,Jennifer Wu,R.D. Young,Brian L. Youngstrom,Paul Wohnoutka,Richard A. Gibbs,Jeffrey Rogers,John G. Hohmann,Michael Hawrylycz,Robert F. Hevner,Zoltán Molnár,John W. Phillips,Chinh Dang,Allan R. Jones,David G. Amaral,Amy Bernard,Ed S. Lein +98 more
TL;DR: A high-resolution transcriptional atlas of rhesus monkey (Macaca mulatta) brain development is described that combines dense temporal sampling of prenatal and postnatal periods with fine anatomical division of cortical and subcortical regions associated with human neuropsychiatric disease.
Journal ArticleDOI
Spatiotemporal transcriptomic divergence across human and macaque brain development.
Ying Zhu,André M. M. Sousa,Tianliuyun Gao,Mario Skarica,Mingfeng Li,Gabriel Santpere,Paula Esteller-Cucala,David Juan,Luis Ferrández-Peral,Forrest O. Gulden,Mo Yang,Daniel J. Miller,Tomas Marques-Bonet,Yuka Imamura Kawasawa,Hongyu Zhao,Nenad Sestan +15 more
TL;DR: This study provides insights into the evolution of gene expression in the developing human brain and may shed some light on potentially human-specific underpinnings of certain neuropsychiatric disorders.
References
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Neural Mechanisms of Selective Visual Attention
Robert Desimone,John S. Duncan +1 more
TL;DR: The two basic phenomena that define the problem of visual attention can be illustrated in a simple example and selectivity-the ability to filter out un wanted information is illustrated.
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The new frontier of genome engineering with CRISPR-Cas9
TL;DR: The power of the CRISPR-Cas9 technology to systematically analyze gene functions in mammalian cells, study genomic rearrangements and the progression of cancers or other diseases, and potentially correct genetic mutations responsible for inherited disorders is illustrated.
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DNA targeting specificity of RNA-guided Cas9 nucleases
Patrick D. Hsu,David A. Scott,David A. Scott,Joshua A. Weinstein,Joshua A. Weinstein,F. Ann Ran,F. Ann Ran,F. Ann Ran,Silvana Konermann,Silvana Konermann,Vineeta Agarwala,Vineeta Agarwala,Vineeta Agarwala,Yinqing Li,Yinqing Li,Eli J. Fine,Xuebing Wu,Ophir Shalem,Ophir Shalem,Thomas J. Cradick,Luciano A. Marraffini,Gang Bao,Feng Zhang,Feng Zhang +23 more
TL;DR: In this article, the Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of single-guide RNAs (sgRNAs) to enable genome editing.
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One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.
Haoyi Wang,Hui Yang,Chikdu S. Shivalila,Meelad M. Dawlaty,Albert W. Cheng,Feng Zhang,Feng Zhang,Rudolf Jaenisch +7 more
TL;DR: The CRISPR/Cas system allows the one-step generation of animals carrying mutations in multiple genes, an approach that will greatly accelerate the in vivo study of functionally redundant genes and of epistatic gene interactions.
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High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.
Yanfang Fu,Jennifer A Foden,Cyd Khayter,Morgan L. Maeder,Deepak Reyon,J. Keith Joung,Jeffry D. Sander +6 more
TL;DR: It is found that single and double mismatches are tolerated to varying degrees depending on their position along the guide RNA (gRNA)-DNA interface, and off-target cleavage of CRISPR-associated (Cas)9-based RGNs is characterized.
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