From a consortium sequence to a unified sequence: The Bacillus subtilis 168 reference genome a decade later
Valérie Barbe,Stéphane Cruveiller,Frank Kunst,Patricia Lenoble,Guillaume Meurice,Agnieszka Sekowska,David Vallenet,Tingzhang Wang,Ivan Moszer,Claudine Médigue,Antoine Danchin +10 more
Reads0
Chats0
TLDR
The updated sequence has been reannotated in agreement with the UniProt protein knowledge base, keeping in perspective the split between the paleome and the cenome, suggesting here that B. subtilis is an epiphyte.Abstract:
Comparative genomics is the cornerstone of identification of gene functions. The immense number of living organisms precludes experimental identification of functions except in a handful of model organisms. The bacterial domain is split into large branches, among which the Firmicutes occupy a considerable space. Bacillus subtilis has been the model of Firmicutes for decades and its genome has been a reference for more than 10 years. Sequencing the genome involved more than 30 laboratories, with different expertises, in a attempt to make the most of the experimental information that could be associated with the sequence. This had the expected drawback that the sequencing expertise was quite varied among the groups involved, especially at a time when sequencing genomes was extremely hard work. The recent development of very efficient, fast and accurate sequencing techniques, in parallel with the development of high-level annotation platforms, motivated the present resequencing work. The updated sequence has been reannotated in agreement with the UniProt protein knowledge base, keeping in perspective the split between the paleome (genes necessary for sustaining and perpetuating life) and the cenome (genes required for occupation of a niche, suggesting here that B. subtilis is an epiphyte). This should permit investigators to make reliable inferences to prepare validation experiments in a variety of domains of bacterial growth and development as well as build up accurate phylogenies.read more
Citations
More filters
Journal ArticleDOI
Condition-Dependent Transcriptome Reveals High-Level Regulatory Architecture in Bacillus subtilis
Pierre Nicolas,Ulrike Mäder,Etienne Dervyn,Tatiana Rochat,Aurélie Leduc,Nathalie Pigeonneau,Elena Bidnenko,Elodie Marchadier,Mark Hoebeke,Stéphane Aymerich,Dörte Becher,Paola Bisicchia,Eric Botella,Olivier Delumeau,Geoff Doherty,Emma L. Denham,Mark J. Fogg,Vincent Fromion,Anne Goelzer,Annette Hansen,Elisabeth Härtig,Colin R. Harwood,Georg Homuth,Hanne Østergaard Jarmer,Matthieu Jules,Edda Klipp,Ludovic Le Chat,François Lecointe,Peter J. Lewis,Wolfram Liebermeister,Anika March,Ruben A. T. Mars,Priyanka Nannapaneni,David Noone,Susanne Pohl,Bernd Rinn,Frank Rügheimer,Praveen K. Sappa,Franck Samson,Marc Schaffer,Benno Schwikowski,Leif Steil,Jörg Stülke,Thomas Wiegert,Kevin M. Devine,Anthony J. Wilkinson,Jan Maarten van Dijl,Michael Hecker,Uwe Völker,Philippe Bessières,Philippe Noirot +50 more
TL;DR: The transcriptomes of Bacillus subtilis exposed to a wide range of environmental and nutritional conditions that the organism might encounter in nature are reported, offering an initial understanding of why certain regulatory strategies may be favored during evolution of dynamic control systems.
Journal ArticleDOI
Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42.
Xiao-Hua Chen,Alexandra Koumoutsi,Romy Scholz,Andreas Eisenreich,Kathrin Schneider,Isabelle Heinemeyer,Burkhard Morgenstern,Björn Voss,Wolfgang R. Hess,Oleg N. Reva,Helmut Junge,Birgit Voigt,Peter R. Jungblut,Joachim Vater,Roderich D. Süssmuth,Heiko Liesegang,Axel Strittmatter,Gerhard Gottschalk,Rainer Borriss +18 more
TL;DR: The B. amyloliquefaciens FZB42 genome reveals an unexpected potential to produce secondary metabolites, including the polyketides bacillaene and difficidin, and identifies four giant gene clusters absent in B. subtilis 168.
Journal ArticleDOI
Whole Genome Phylogeny of Bacillus by Feature Frequency Profiles (FFP)
Aisuo Wang,Gavin Ash +1 more
TL;DR: Fifty complete Bacillus genome sequences and associated plasmids were compared using the “feature frequency profile” (FFP) method and the resulting whole-genome phylogeny supports the placement of three Bacillus species as a single clade.
Journal ArticleDOI
Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group.
Simon Caulier,Catherine Nannan,Annika Gillis,Florent Licciardi,Claude Bragard,Jacques Mahillon +5 more
TL;DR: A comprehensive way to visualize the antimicrobial spectrum described within the B. subtilis group is suggested, which distinguishes the bioactive metabolites based on their biosynthetic pathways and chemical nature: i.e., ribosomal peptides (RPs), volatile compounds, polyketides (PKs), non-ribosomal proteins (NRPs), and hybrids between PKs and NRPs.
Journal ArticleDOI
Bacillus subtilis: from soil bacterium to super-secreting cell factory.
TL;DR: The engineering of B. subtilis into a next-generation super-secreting cell factory requires combined Systems and Synthetic Biology approaches and can be optimized from the single molecule to the network level while, at the same time, taking into account the balanced use of cellular resources.
References
More filters
Book
Bergey's Manual of Systematic Bacteriology
TL;DR: BCL3 and Sheehy cite Bergey's manual of determinative bacteriology of which systematic bacteriology, first edition, is an expansion.
Journal ArticleDOI
Genome sequencing in microfabricated high-density picolitre reactors
Marcel Margulies,Michael Egholm,William E. Altman,Said Attiya,Joel S. Bader,Lisa A. Bemben,Jan Berka,Michael S. Braverman,Yi-Ju Chen,Zhoutao Chen,Scott Dewell,Lei Du,J. M. Fierro,Xavier V. Gomes,Brian C. Godwin,Wen He,Scott Edward Helgesen,Chun Heen Ho,Gerard P. Irzyk,Szilveszter C. Jando,Maria L. I. Alenquer,Thomas P. Jarvie,Kshama B. Jirage,Jong-Bum Kim,James R. Knight,Janna R. Lanza,John H. Leamon,Steven Lefkowitz,Ming Lei,Jing Li,Kenton Lohman,Hong Lu,Vinod Makhijani,Keith Mcdade,Michael P. McKenna,Eugene W. Myers,Elizabeth Nickerson,John Nobile,Ramona Plant,Bernard P. Puc,Michael T. Ronan,George T. Roth,Gary J. Sarkis,Jan Fredrik Simons,John Simpson,Maithreyan Srinivasan,Karrie R. Tartaro,Alexander Tomasz,Kari A. Vogt,Greg A. Volkmer,Shally H. Wang,Yong Wang,Michael P. Weiner,Pengguang Yu,Richard F. Begley,Jonathan M. Rothberg +55 more
TL;DR: A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.
Journal ArticleDOI
Versatile and open software for comparing large genomes
Stefan Kurtz,Adam M. Phillippy,Arthur L. Delcher,Michael E. Smoot,Martin Shumway,Corina Antonescu,Steven L. Salzberg +6 more
TL;DR: The newest version of MUMmer easily handles comparisons of large eukaryotic genomes at varying evolutionary distances, as demonstrated by applications to multiple genomes.
Journal ArticleDOI
The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
F. Kunst,Naotake Ogasawara,Ivan Moszer,Alessandra M. Albertini,G. Alloni,Vasco Azevedo,M. G. Bertero,M. G. Bertero,Philippe Bessières,Bolotin Ap,S. Borchert,Rainer Borriss,L. Boursier,Alain Brans,M. Braun,S. C. Brignell,Sierd Bron,S. Brouillet,S. Brouillet,Carlo V. Bruschi,B. Caldwell,V. Capuano,Noel Carter,Soo Keun Choi,J.-J. Codani,Ian F. Connerton,Nicola J. Cummings,Richard A. Daniel,François Denizot,Kevin M. Devine,A. Düsterhöft,Stanislav Dusko Ehrlich,P. T. Emmerson,K. D. Entian,Jeff Errington,C. Fabret,Eugenio Ferrari,D. Foulger,C. Fritz,Masaya Fujita,Yasutaro Fujita,S. Fuma,Alessandro Galizzi,Nathalie Galleron,Sa Youl Ghim,Philippe Glaser,André Goffeau,E. J. Golightly,Guido Grandi,G. Guiseppi,BJ Guy,Kazuko Haga,Jacques Haiech,Colin R. Harwood,Alain Hénaut,H. Hilbert,Siger Holsappel,S. Hosono,Marie-Françoise Hullo,Mitsuhiro Itaya,Louis M. Jones,Bernard Joris,Dimitri Karamata,Y. Kasahara,M. Klaerr-Blanchard,Carsten Klein,Y. Kobayashi,P. Koetter,G. Koningstein,Susanne Krogh,Miyuki Kumano,Kanako Kurita,Alla Lapidus,S. Lardinois,J. Lauber,Vladimir Lazarevic,Simon Ming-Yuen Lee,Alain Levine,H. Liu,S. Masuda,Catherine Mauël,Claudine Médigue,Claudine Médigue,N. Medina,Rafael P. Mellado,Motoki Mizuno,D. Moestl,S. Nakai,Michiel A. Noback,David Noone,Mary O'Reilly,K. Ogawa,A. Ogiwara,B. Oudega,S.-H. Park,Victor Parro,Thomas Pohl,Daniel Portetelle,Steffen Porwollik,A. M. Prescott,E. Presecan,Petar Pujic,Bénédicte Purnelle,Georges Rapoport,M. Rey,Stacey Reynolds,Michael A. Rieger,Carlo Rivolta,Eduardo P. C. Rocha,Eduardo P. C. Rocha,B. Roche,Matthias Rose,Yoshito Sadaie,Toshitada Sato,E. Scanlan,S. Schleich,R. Schroeter,F Scoffone,Junichi Sekiguchi,Agnieszka Sekowska,Simone J. Séror,Pascale Serror,B.-S. Shin,Blazenka Soldo,Alexei Sorokin,E. Tacconi,T. Takagi,Hideyuki Takahashi,Ken-Ichi Takemaru,Michio Takeuchi,A. Tamakoshi,Tetsu Tanaka,Peter Terpstra,Angelo Tognoni,Valentina Tosato,Shigeki Uchiyama,Micheline Vandenbol,Françoise Vannier,A. Vassarotti,Alain Viari,R. Wambutt,E. Wedler,H. Wedler,T. Weitzenegger,P. Winters,Anil Wipat,Hiroki Yamamoto,Kunio Yamane,K. Yasumoto,Katsunori Yata,K. Yoshida,Hisashi Yoshikawa,Emmanuelle Zumstein,Hiroshi Yoshikawa,Antoine Danchin +154 more
TL;DR: Bacillus subtilis is the best-characterized member of the Gram-positive bacteria, indicating that bacteriophage infection has played an important evolutionary role in horizontal gene transfer, in particular in the propagation of bacterial pathogenesis.
Journal ArticleDOI
Consed: A Graphical Tool for Sequence Finishing
TL;DR: A finishing tool, consed, which attempts to implement principles of shotgun sequencing by using error probabilities from phred and phrap as an objective criterion to guide the entire finishing process.
Related Papers (5)
The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
F. Kunst,Naotake Ogasawara,Ivan Moszer,Alessandra M. Albertini,G. Alloni,Vasco Azevedo,M. G. Bertero,M. G. Bertero,Philippe Bessières,Bolotin Ap,S. Borchert,Rainer Borriss,L. Boursier,Alain Brans,M. Braun,S. C. Brignell,Sierd Bron,S. Brouillet,S. Brouillet,Carlo V. Bruschi,B. Caldwell,V. Capuano,Noel Carter,Soo Keun Choi,J.-J. Codani,Ian F. Connerton,Nicola J. Cummings,Richard A. Daniel,François Denizot,Kevin M. Devine,A. Düsterhöft,Stanislav Dusko Ehrlich,P. T. Emmerson,K. D. Entian,Jeff Errington,C. Fabret,Eugenio Ferrari,D. Foulger,C. Fritz,Masaya Fujita,Yasutaro Fujita,S. Fuma,Alessandro Galizzi,Nathalie Galleron,Sa Youl Ghim,Philippe Glaser,André Goffeau,E. J. Golightly,Guido Grandi,G. Guiseppi,BJ Guy,Kazuko Haga,Jacques Haiech,Colin R. Harwood,Alain Hénaut,H. Hilbert,Siger Holsappel,S. Hosono,Marie-Françoise Hullo,Mitsuhiro Itaya,Louis M. Jones,Bernard Joris,Dimitri Karamata,Y. Kasahara,M. Klaerr-Blanchard,Carsten Klein,Y. Kobayashi,P. Koetter,G. Koningstein,Susanne Krogh,Miyuki Kumano,Kanako Kurita,Alla Lapidus,S. Lardinois,J. Lauber,Vladimir Lazarevic,Simon Ming-Yuen Lee,Alain Levine,H. Liu,S. Masuda,Catherine Mauël,Claudine Médigue,Claudine Médigue,N. Medina,Rafael P. Mellado,Motoki Mizuno,D. Moestl,S. Nakai,Michiel A. Noback,David Noone,Mary O'Reilly,K. Ogawa,A. Ogiwara,B. Oudega,S.-H. Park,Victor Parro,Thomas Pohl,Daniel Portetelle,Steffen Porwollik,A. M. Prescott,E. Presecan,Petar Pujic,Bénédicte Purnelle,Georges Rapoport,M. Rey,Stacey Reynolds,Michael A. Rieger,Carlo Rivolta,Eduardo P. C. Rocha,Eduardo P. C. Rocha,B. Roche,Matthias Rose,Yoshito Sadaie,Toshitada Sato,E. Scanlan,S. Schleich,R. Schroeter,F Scoffone,Junichi Sekiguchi,Agnieszka Sekowska,Simone J. Séror,Pascale Serror,B.-S. Shin,Blazenka Soldo,Alexei Sorokin,E. Tacconi,T. Takagi,Hideyuki Takahashi,Ken-Ichi Takemaru,Michio Takeuchi,A. Tamakoshi,Tetsu Tanaka,Peter Terpstra,Angelo Tognoni,Valentina Tosato,Shigeki Uchiyama,Micheline Vandenbol,Françoise Vannier,A. Vassarotti,Alain Viari,R. Wambutt,E. Wedler,H. Wedler,T. Weitzenegger,P. Winters,Anil Wipat,Hiroki Yamamoto,Kunio Yamane,K. Yasumoto,Katsunori Yata,K. Yoshida,Hisashi Yoshikawa,Emmanuelle Zumstein,Hiroshi Yoshikawa,Antoine Danchin +154 more
Condition-Dependent Transcriptome Reveals High-Level Regulatory Architecture in Bacillus subtilis
Pierre Nicolas,Ulrike Mäder,Etienne Dervyn,Tatiana Rochat,Aurélie Leduc,Nathalie Pigeonneau,Elena Bidnenko,Elodie Marchadier,Mark Hoebeke,Stéphane Aymerich,Dörte Becher,Paola Bisicchia,Eric Botella,Olivier Delumeau,Geoff Doherty,Emma L. Denham,Mark J. Fogg,Vincent Fromion,Anne Goelzer,Annette Hansen,Elisabeth Härtig,Colin R. Harwood,Georg Homuth,Hanne Østergaard Jarmer,Matthieu Jules,Edda Klipp,Ludovic Le Chat,François Lecointe,Peter J. Lewis,Wolfram Liebermeister,Anika March,Ruben A. T. Mars,Priyanka Nannapaneni,David Noone,Susanne Pohl,Bernd Rinn,Frank Rügheimer,Praveen K. Sappa,Franck Samson,Marc Schaffer,Benno Schwikowski,Leif Steil,Jörg Stülke,Thomas Wiegert,Kevin M. Devine,Anthony J. Wilkinson,Jan Maarten van Dijl,Michael Hecker,Uwe Völker,Philippe Bessières,Philippe Noirot +50 more
Essential Bacillus subtilis genes
Kazuto Kobayashi,Stanislav Dusko Ehrlich,Alessandra M. Albertini,G. Amati,Kasper Krogh Andersen,Maryvonne Arnaud,Kei Asai,S. Ashikaga,Stéphane Aymerich,Philippe Bessières,F. Boland,S.C. Brignell,Sierd Bron,Keigo Bunai,J. Chapuis,L.C. Christiansen,Antoine Danchin,Michel Débarbouillé,Etienne Dervyn,E. Deuerling,Kevin M. Devine,Susanne Krogh Devine,Oliver Dreesen,Jeffery Errington,Sabine Fillinger,Simon J. Foster,Yasutaro Fujita,Alessandro Galizzi,Rozenn Gardan,Caroline Eschevins,Tatsuya Fukushima,Kazuko Haga,Colin R. Harwood,Michael Hecker,D. Hosoya,Marie-Françoise Hullo,Hiroshi Kakeshita,Dimitri Karamata,Yasuhiro Kasahara,Fujio Kawamura,K. Koga,P. Koski,Ritsuko Kuwana,Daisuke Imamura,M. Ishimaru,Shu Ishikawa,I. Ishio,D. Le Coq,Anne Masson,Catherine Mauël,Rob Meima,Rafael P. Mellado,Anne Moir,Shigeki Moriya,E. Nagakawa,Hideaki Nanamiya,S. Nakai,Per Nygaard,Mitsuo Ogura,T. Ohanan,Mary O'Reilly,M. O'Rourke,Zoltán Prágai,H.M. Pooley,Georges Rapoport,J.P. Rawlins,L.A. Rivas,Carlo Rivolta,A. Sadaie,Yoshito Sadaie,Matti Sarvas,T. Sato,Hans Henrik Saxild,E. Scanlan,Wolfgang Schumann,J.F.M.L. Seegers,Junichi Sekiguchi,Agnieszka Sekowska,Simone J. Séror,M. Simon,P. Stragier,R. Studer,Hiromu Takamatsu,Teruo Tanaka,M. Takeuchi,H.B. Thomaides,Valerie Vagner,J.M. van Dijl,Kazuhito Watabe,Anil Wipat,Hiroki Yamamoto,M. Yamamoto,Y. Yamamoto,Kunio Yamane,Katsunori Yata,K. Yoshida,Hisashi Yoshikawa,Ulrich Zuber,Naotake Ogasawara +98 more