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Open AccessJournal ArticleDOI

NIH Image to ImageJ: 25 years of image analysis

TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.
Abstract
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.

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Journal ArticleDOI

Pickering emulsions by combining cellulose nanofibrils and nanocrystals: phase behavior and depletion stabilization

TL;DR: In this paper, cellulose nanofibrils (CNF) were used to induce depletion stabilization of oil-in-water Pickering emulsions formed by interfacial adsorption of another type of nanocellulose, namely, cellulose nano-stals (CNC).
Book ChapterDOI

ASC speck formation as a readout for inflammasome activation.

TL;DR: This work describes the generation of a clonal inflammasome reporter macrophage cell line overexpressing fluorescently tagged ASC and discusses stimulation conditions and the microscopic evaluation of ASC speck formation.
Journal ArticleDOI

The extracellular matrix Component Psl provides fast-acting antibiotic defense in Pseudomonas aeruginosa biofilms.

TL;DR: In this paper, a combination of static and continuous-flow biofilm experiments was used to show that Psl, one major polysaccharide in the Pseudomonas aeruginosa biofilm matrix, provides a generic first line of defense toward antibiotics with diverse biochemical properties during the initial stages of biofilm development.
Journal ArticleDOI

Heme oxygenase-1 mitigates ferroptosis in renal proximal tubule cells

TL;DR: C Cotreatment with ferrostatin-1 (ferroptosis inhibitor), deferoxamine (iron chelator), or N-acetyl-l-cysteine (glutathione replenisher) significantly increased cell viability and attenuated erastin-induced ferroPTosis in both HO-1+/+ and HO- 1-/- PTCs.
Journal ArticleDOI

Discovery of Unconventional Kinetochores in Kinetoplastids

TL;DR: In this paper, the authors reported the identification of 19 kinetochore proteins (KKT1-19) in Trypanosoma brucei and found that the majority of them were conserved among kinetoplastids.
References
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Journal ArticleDOI

Fiji: an open-source platform for biological-image analysis

TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
Journal ArticleDOI

CellProfiler: image analysis software for identifying and quantifying cell phenotypes

TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI

ImageJ for microscopy

TL;DR: The near-comprehensive range of import filters that allow easy access to image and meta-data, a broad suite processing and analysis routine, and enthusiastic support from a friendly mailing list are invaluable for all microscopy labs and facilities-not just those on a budget.
Journal ArticleDOI

Computer control of microscopes using µManager.

TL;DR: This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.
Journal ArticleDOI

Icy: an open bioimage informatics platform for extended reproducible research

TL;DR: Icy is a collaborative bioimage informatics platform that combines a community website for contributing and sharing tools and material, and software with a high-end visual programming framework for seamless development of sophisticated imaging workflows.
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