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NIH Image to ImageJ: 25 years of image analysis

TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.
Abstract
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.

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Hierarchical patterning modes orchestrate hair follicle morphogenesis.

TL;DR: A hierarchy of periodic patterning modes operating in organogenesis is illustrated, finding that mesenchymal cell condensation at hair follicles is locally directed by an epidermal prepattern, and a latent dermal capacity to undergo spatially patterned self-organisation in the absence of epithelial direction.
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AKT1 Activation Promotes Development of Melanoma Metastases

TL;DR: It is demonstrated that AKT1 activation is sufficient to elicit lung and brain metastases in this context and revealed that activation ofAKT1 is distinct from PTEN silencing in metastatic melanoma progression.
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Microplastics and other harmful substances released from disposable paper cups into hot water.

TL;DR: In this article, the degradation of polyethylene and copolymers was evaluated as a result of exposure to hot water (85-90°C) and heavy metals like Pb, Cr, and Cd were detected in the films which can be transferred into hot water.
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MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

TL;DR: A family of six cyanine-based fluorescent turn-on PM probes that emit from orange to near infrared when reaching the PM, and enable homogeneous and selective PM staining with excellent contrast in mono- and two-photon microscopy, constitute a universal toolkit for cell biology and neuroscience biomembrane imaging with a variety of microscopy techniques.
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Apical constriction initiates new bud formation during monopodial branching of the embryonic chicken lung.

TL;DR: It is shown that monopodial branching is initiated by apical constriction of the airway epithelium, and not by differential cell proliferation, using computational modeling and quantitative imaging of embryonic chicken lung explants.
References
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Journal ArticleDOI

Fiji: an open-source platform for biological-image analysis

TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes

TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
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ImageJ for microscopy

TL;DR: The near-comprehensive range of import filters that allow easy access to image and meta-data, a broad suite processing and analysis routine, and enthusiastic support from a friendly mailing list are invaluable for all microscopy labs and facilities-not just those on a budget.
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Computer control of microscopes using µManager.

TL;DR: This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.
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Icy: an open bioimage informatics platform for extended reproducible research

TL;DR: Icy is a collaborative bioimage informatics platform that combines a community website for contributing and sharing tools and material, and software with a high-end visual programming framework for seamless development of sophisticated imaging workflows.
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