NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
Citations
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Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison.
Eva Wegel,Antonia Gohler,B C Lagerholm,Alan Wainman,Stephan Uphoff,Rainer Kaufmann,Rainer Kaufmann,Ian M. Dobbie +7 more
TL;DR: In this article, the authors compare the individual strengths and weaknesses of SIM, STED, and SMLM by imaging a variety of different subcellular structures in fixed cells.
Journal ArticleDOI
The Arabidopsis Cellulose Synthase Complex: A Proposed Hexamer of CESA Trimers in an Equimolar Stoichiometry
TL;DR: A 1:1:1 stoichiometry is shown between the three Arabidopsis thaliana secondary cell wall isozymes, a crucial advance in understanding how CESAs integrate to form higher order complexes, which is a key determinate of cellulose microfibril and cell wall properties.
Journal ArticleDOI
Breaking the selectivity-uptake trade-off of photoimmunoconjugates with nanoliposomal irinotecan for synergistic multi-tier cancer targeting
Barry J. Liang,Michael Pigula,Yan Baglo,Daniel Najafali,Tayyaba Hasan,Tayyaba Hasan,Huang-Chiao Huang,Huang-Chiao Huang,Huang-Chiao Huang +8 more
TL;DR: A novel photoimmunoconjugate-nanoliposome (PIC-Nal-IRI) comprising of three clinically used agents offers a promising strategy to overcome the selectivity-uptake trade-off, improvephotoimmunotherapy efficacy, and enable multi-tier cancer targeting.
Journal ArticleDOI
A High-Throughput Organoid Microinjection Platform to Study Gastrointestinal Microbiota and Luminal Physiology.
Ian A. Williamson,Jason W. Arnold,Leigh Ann Samsa,Liam T. Gaynor,Matthew DiSalvo,Jordan L. Cocchiaro,Ian M. Carroll,M. Andrea Azcarate-Peril,John F. Rawls,Nancy L. Allbritton,Scott T. Magness +10 more
TL;DR: Development and validation of a high-throughput organoid microinjection system for cargo delivery to the organoid lumen and high-content sampling and the requirement to normalize for organoid growth for accurate assessment of barrier function are reported.
Journal ArticleDOI
Structure/property (constitutive and spallation response) of additively manufactured 316L stainless steel
George T. Gray,Veronica Livescu,Paulo Rigg,Carl P. Trujillo,Carl M. Cady,S.R. Chen,John S. Carpenter,Thomas J. Lienert,Saryu Fensin +8 more
TL;DR: In this paper, cylindrical samples of 316L SS were produced using a LENS MR-7 laser additive manufacturing system from Optomec (Albuquerque, NM) equipped with a 1.kW Yb-fiber laser.
References
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