NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
Citations
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Journal ArticleDOI
Golden single-atomic-site platinum electrocatalysts
Paul N. Duchesne,Ziyou Li,Christopher P. Deming,Victor Fung,Xiaojing Zhao,Jun Yuan,Tom Regier,Ali Aldalbahi,Zainab M. Almarhoon,Shaowei Chen,De-en Jiang,Nanfeng Zheng,Peng Zhang +12 more
TL;DR: Extensive structural characterization and theoretical density functional theory simulations of the best-performing catalysts revealed densely packed single-atom Pt surface sites surrounded by Au atoms, which suggests that their superior catalytic activity and selectivity could be attributed to the unique structural and alloy-bonding properties of these single-atomic-site catalysts.
Journal ArticleDOI
Video-rate volumetric functional imaging of the brain at synaptic resolution
Rongwen Lu,Wenzhi Sun,Yajie Liang,Aaron M. Kerlin,Jens Bierfeld,Johannes D. Seelig,Daniel E. Wilson,Benjamin Scholl,Boaz Mohar,Masashi Tanimoto,Masashi Tanimoto,Minoru Koyama,David Fitzpatrick,Michael B. Orger,Na Ji +14 more
TL;DR: An optical module is presented that is easily integrated into standard two-photon laser-scanning microscopes to generate an axially elongated Bessel focus, which when scanned in 2D turns frame rate into volume rate.
Journal ArticleDOI
A chemoproteomic platform to quantitatively map targets of lipid-derived electrophiles.
TL;DR: A competitive activity–based profiling method is described for quantifying the reactivity of electrophilic compounds against >1,000 cysteines in parallel in the human proteome and shows that one of these proteins, ZAK kinase, is labeled by HNE on a conserved, active site–proximal cysteine and that the resulting enzyme inhibition creates a negative feedback mechanism that can suppress the activation of JNK pathways normally induced by oxidative stress.
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GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown root systems
Rubén Rellán-Álvarez,Guillaume Lobet,Heike Lindner,Pierre-Luc M Pradier,Jose Sebastian,Muh-Ching Yee,Yu Geng,Charlotte Trontin,Therese LaRue,Amanda Schrager-Lavelle,Cara H. Haney,Rita Nieu,Julin N. Maloof,John P. Vogel,José R. Dinneny +14 more
TL;DR: This work has developed image analysis algorithms that allow the spatial integration of soil properties, gene expression, and root system architecture traits and proposes GLO-Roots as a system that has great utility in presenting environmental stimuli to roots in ways that evoke natural adaptive responses and in providing tools for studying the multi-dimensional nature of such processes.
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Short hairpin RNA against PTEN enhances regenerative growth of corticospinal tract axons after spinal cord injury.
TL;DR: Insight is revealed into the critical extrinsic and intrinsic regulators of axon regeneration and shRNA is established as a viable means to manipulate these regulators and translate findings into other mammalian models.
References
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