NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
Citations
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Next-generation computational tools for interrogating cancer immunity.
TL;DR: Methods — primarily computational tools — for characterizing diverse aspects of cancer–immune cell interactions, including antigen presentation, T cell repertoires and heterogeneity in cell types and cell states are discussed.
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Netrin-1 Promotes Excitatory Synaptogenesis between Cortical Neurons by Initiating Synapse Assembly
Jennifer S. Goldman,Jennifer S. Goldman,Jennifer S. Goldman,Mohammed A. Ashour,Margaret H. Magdesian,Nicolas X. Tritsch,Stephanie N. Harris,Nicolas Christofi,Raja Chemali,Yaakov E. Stern,Greta Thompson-Steckel,Greta Thompson-Steckel,Pavel Gris,Stephen D. Glasgow,Peter Grutter,Jean-François Bouchard,Edward S. Ruthazer,Edward S. Ruthazer,David Stellwagen,Timothy E. Kennedy,Timothy E. Kennedy +20 more
TL;DR: It is demonstrated that netrin-1 increases the number and strength of excitatory synapses made between developing cortical neurons and increases the frequency and amplitude of mEPSCs recorded from cortical pyramidal neurons.
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Frequent exchange of the DNA polymerase during bacterial chromosome replication
Thomas R. Beattie,Nitin Kapadia,Emilien Nicolas,Stephan Uphoff,Adam J. M. Wollman,Mark C. Leake,Rodrigo Reyes-Lamothe +6 more
TL;DR: By examining replisomes in live E. coli with fluorescence microscopy, it is found that the Pol III* subassembly frequently disengages from the replisome during DNA synthesis and exchanges with free copies from solution, supporting a fully discontinuous mechanism in which synthesis of both leading and lagging strands is frequently interrupted.
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Microstructural evolution and mechanical properties of differently heat-treated binder jet printed samples from gas- and water-atomized alloy 625 powders
TL;DR: In this paper, the effect of powders resulting from different atomization methods on properties of binder jet printed and heat-treated samples was investigated, and the results showed that the difference in microstructural evolution results in different mechanical properties.
Journal ArticleDOI
Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage
Jonathan M. Brunger,Nguyen P.T. Huynh,Caitlin M. Guenther,Pablo Perez-Pinera,Franklin T. Moutos,Johannah Sanchez-Adams,Charles A. Gersbach,Farshid Guilak +7 more
TL;DR: A self-contained bioactive scaffold capable of mediating stem cell differentiation and formation of a cartilaginous extracellular matrix (ECM) using a lentivirus-based method is generated and opens new avenues in the development of bioactive implants that circumvent the need for ex vivo tissue generation by enabling the long-term goal of in situ tissue engineering.
References
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