NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
Citations
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Rubisco is a small fraction of total protein in marine phytoplankton
TL;DR: It is suggested that phytoplankton contain the minimum concentration of enzyme necessary to support observed growth rates, and Ribulose 1,5 bisphosphate carboxylase oxygenase (Rubisco) concentrations were quantified as a proportion of total protein in eight species of microalgae.
Journal ArticleDOI
Self-organization of human embryonic stem cells on micropatterns
Alessia Deglincerti,Fred Etoc,M Cecilia Guerra,Iain Martyn,Jakob J. Metzger,Albert Ruzo,Mijo Simunovic,Anna Yoney,Ali H. Brivanlou,Eric D. Siggia,Aryeh Warmflash +10 more
TL;DR: A micropatterning approach in which human embryonic stem cells are confined to disk-shaped, submillimeter colonies, which describes a robust platform for quantitative analysis of the mechanisms associated with pattern formation at the onset of gastrulation.
Journal ArticleDOI
Ubiquinone accumulation improves osmotic-stress tolerance in Escherichia coli
Daniel C. Sévin,Uwe Sauer +1 more
TL;DR: Besides regulating intracellular osmolality, E. coli enhances its cytoplasmic membrane stability to withstand osmotic stress, and in vitro experiments with artificial liposomes showed that mechanical membrane stabilization is a principal mechanism of Q8-mediated osmoprotection.
Journal ArticleDOI
An autoinhibited structure of α-catenin and its implications for vinculin recruitment to adherens junctions.
Noboru Ishiyama,Nobutoshi Tanaka,Kentaro Abe,Yoo Jeong Yang,Yazan M. Abbas,Masataka Umitsu,Bhushan Nagar,Stephanie A. Bueler,John L. Rubinstein,Masatoshi Takeichi,Mitsuhiko Ikura,Mitsuhiko Ikura +11 more
TL;DR: A molecular switch mechanism involving multistate conformational changes of α-catenin is proposed, driven by actomyosin-generated tension to dynamically regulate the vinculin-assisted linkage between adherens junctions and the actin cytoskeleton.
Journal ArticleDOI
Eighteen years of ecological monitoring reveals multiple lines of evidence for tundra vegetation change
Isla H. Myers-Smith,Meagan M. Grabowski,Haydn J.D. Thomas,Sandra Angers-Blondin,Gergana N. Daskalova,Anne D. Bjorkman,Anne D. Bjorkman,Andrew M. Cunliffe,Jakob J. Assmann,Joseph S. Boyle,Edward McLeod,Samuel McLeod,Ricky Joe,Paden Lennie,Deon Arey,Richard R. Gordon,Cameron D. Eckert +16 more
TL;DR: In this paper, the authors present the findings of a collaboration between government scientists, local people, park rangers, and academic researchers that provide insights into changes in plant composition, phenology, and growth over 18 years on Qikiqtaruk-Herschel Island, Canada.
References
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Icy: an open bioimage informatics platform for extended reproducible research
Fabrice de Chaumont,Stephane Dallongeville,Nicolas Chenouard,Nicolas Hervé,Sorin Pop,Thomas Provoost,Vannary Meas-Yedid,Praveen Pankajakshan,Timothee Lecomte,Yoann Le Montagner,Thibault Lagache,Alexandre Dufour,Jean-Christophe Olivo-Marin +12 more
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