NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
Citations
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The influence of nanostructured features on bacterial adhesion and bone cell functions on severely shot peened 316L stainless steel
Sara Bagherifard,Sara Bagherifard,Sara Bagherifard,Daniel J. Hickey,Alba C. de Luca,Vera Malheiro,Athina E. Markaki,Mario Guagliano,Thomas J. Webster +8 more
TL;DR: This study demonstrated the advantages of the proposed shot peening treatment to produce multifunctional 316L stainless steel materials for improved implant functions without necessitating the use of drugs.
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Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration.
Choun-Sea Lin,Chen-Tran Hsu,Ling-Hung Yang,Lan-Ying Lee,Jin-Yuan Fu,Qiao-Wei Cheng,Fu-Hui Wu,Han C.W. Hsiao,Yesheng Zhang,Ru Zhang,Wan-Jung Chang,Chen-Ting Yu,Wen Wang,Li-Jen Liao,Stanton B. Gelvin,Ming-Che Shih +15 more
TL;DR: Improved the process of protoplast isolation and transfection of several plant species and developed a method to regenerate single mutagenized Nicotianna tabacum protoplasts into mature plants for targeted mutagenesis of the phytoene desaturase gene.
Journal ArticleDOI
Grading the neuroendocrine tumors of the lung: an evidence-based proposal
Guido Rindi,Catherine Klersy,Frediano Inzani,Giovanni Fellegara,Luca Ampollini,Andrea Ardizzoni,Nicoletta Campanini,Paolo Carbognani,T. De Pas,Domenico Galetta,P L Granone,Luisella Righi,Michele Rusca,Lorenzo Spaggiari,Marcello Tiseo,Giuseppe Viale,Marco Volante,Mauro Papotti,Giuseppe Pelosi +18 more
TL;DR: A three-tiers grading system based on Ki67 index, mitotic count, and necrosis with cutoffs specifically generated for lung neuroendocrine tumors is prognostically effective and accurate.
Journal ArticleDOI
PlantCV v2: Image analysis software for high-throughput plant phenotyping
Malia A. Gehan,Noah Fahlgren,Arash Abbasi,Jeffrey C. Berry,Steven T. Callen,Steven T. Callen,Leonardo Chavez,Andrew N. Doust,Max J. Feldman,Kerrigan B. Gilbert,John G. Hodge,J. Steen Hoyer,J. Steen Hoyer,Andy Lin,Suxing Liu,Suxing Liu,Cesar Lizarraga,Argelia Lorence,Michael Miller,Michael Miller,Eric Platon,Monica Tessman,Monica Tessman,Tony Sax +23 more
TL;DR: New functionality in the second major release of PlantCV includes a set of new image processing and normalization tools, support for analyzing images that include multiple plants, leaf segmentation, landmark identification tools for morphometrics, and modules for machine learning.
Journal ArticleDOI
Defined p16High senescent cell types are indispensable for mouse healthspan
Laurent Grosse,Nicole Wagner,Alexander Emelyanov,Clement Molina,Sandra Lacas-Gervais,Kay-Dietrich Wagner,Dmitry V. Bulavin +6 more
TL;DR: It is found that age-induced p16High senescence is a slow process that manifests around 10-12 months of age and senescent LSECs are not replaced after removal and have important structural and functional roles in the aging organism.
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