NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
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Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche.
Yue Shao,Kenichiro Taniguchi,Katherine Gurdziel,Ryan F. Townshend,Xufeng Xue,Koh Meng Aw Yong,Jianming Sang,Jason R. Spence,Deborah L. Gumucio,Jianping Fu +9 more
TL;DR: This study unveils the self-organizing and mechanosensitive nature of human amniogenesis and establishes the first hPSC-based model for investigating peri-implantation human amnion development, thereby helping advance human embryology and reproductive medicine.
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What Is Trophoblast? A Combination of Criteria Define Human First-Trimester Trophoblast
Cheryl Q.E. Lee,Lucy Gardner,Margherita Y. Turco,Nancy Q. Zhao,Matthew J. Murray,Nicholas Coleman,Janet Rossant,Myriam Hemberger,Ashley Moffett +8 more
TL;DR: This study identifies a robust panel, including both protein and non-protein-coding markers that, in combination, can be used to reliably define cells as characteristic of early trophoblast.
Journal ArticleDOI
Fatty Acid Oxidation Mediated by Acyl-CoA Synthetase Long Chain 3 Is Required for Mutant KRAS Lung Tumorigenesis
Mahesh S. Padanad,Georgia Konstantinidou,Niranjan Venkateswaran,Margherita Melegari,Smita Rindhe,Matthew A. Mitsche,Chendong Yang,Kimberly Batten,Kenneth E. Huffman,Jingwen Liu,Ximing Tang,Jaime Rodriguez-Canales,Neda Kalhor,Jerry W. Shay,John D. Minna,Jeffrey G. McDonald,Ignacio I. Wistuba,Ralph J. DeBerardinis,Pier Paolo Scaglioni +18 more
TL;DR: It is reported that mutant KRAS regulates intracellular fatty acid metabolism through Acyl-coenzyme A (CoA) synthetase long-chain family member 3 (ACSL3), which converts fatty acids into fatty A cylinder-CoA esters, the substrates for lipid synthesis and β-oxidation.
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Resident Macrophages Mediate Islet Amyloid Polypeptide–Induced Islet IL-1β Production and β-Cell Dysfunction
TL;DR: Data provide the first evidence that IAPP aggregates skew resident islet macrophages toward a proinflammatory phenotype and suggest a mechanism by which anti-inflammatory therapies may protect β-cells from IAPP-induced islet dysfunction.
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A Bright Fluorescent Probe for H2S Enables Analyte-Responsive, 3D Imaging in Live Zebrafish Using Light Sheet Fluorescence Microscopy
Matthew D. Hammers,Michael J. Taormina,Matthew M. Cerda,Leticia A. Montoya,Daniel T. Seidenkranz,Raghuveer Parthasarathy,Michael D. Pluth +6 more
TL;DR: An azide-functionalized O-methylrhodol fluorophore, MeRho-Az, which exhibits a rapid >1000-fold fluorescence response when treated with H2S, is selective for H 2S over other biological analytes, and has a detection limit of 86 nM is developed.
References
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