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NIH Image to ImageJ: 25 years of image analysis

TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.
Abstract
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.

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Sewage sludge application as a vehicle for microplastics in eastern Spanish agricultural soils

TL;DR: Qualification of microplastic pollution in agricultural fields through the application of sewage sludge is largely unknown, and soils' plastic loads increased by 280 light density microplastics kg-1 and 430 heavy densitymicroplasticskg-1 with each successive application of Sewage sludge, indicating that wastewater sludge application results in accumulation of micro Plastics in agricultural soils.
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Lost in Transition: Start-Up of Glycolysis Yields Subpopulations of Nongrowing Cells

TL;DR: How cell fate can be determined by glycolytic dynamics combined with cell heterogeneity purely at the metabolic level is revealed, extending fundamental knowledge of this central pathway that is dysfunctional in diseases such as diabetes and cancer.
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Genome-guided design of a defined mouse microbiota that confers colonization resistance against Salmonella enterica serovar Typhimurium

TL;DR: A collection of murine bacterial strains and a modular design approach are used to create a minimal bacterial community that provided colonization resistance against the human enteric pathogen Salmonella enterica serovar Typhimurium and established a highly versatile experimental system that showed efficacy in an enteric infection model.
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Cell to whole-plant phenotyping: the best is yet to come.

TL;DR: Overall, attention is focused on spatial and temporal resolution because these are crucial aspects of imaging procedures in plant phenotyping systems.
References
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Journal ArticleDOI

Fiji: an open-source platform for biological-image analysis

TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes

TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI

ImageJ for microscopy

TL;DR: The near-comprehensive range of import filters that allow easy access to image and meta-data, a broad suite processing and analysis routine, and enthusiastic support from a friendly mailing list are invaluable for all microscopy labs and facilities-not just those on a budget.
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Computer control of microscopes using µManager.

TL;DR: This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.
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Icy: an open bioimage informatics platform for extended reproducible research

TL;DR: Icy is a collaborative bioimage informatics platform that combines a community website for contributing and sharing tools and material, and software with a high-end visual programming framework for seamless development of sophisticated imaging workflows.
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