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NIH Image to ImageJ: 25 years of image analysis

TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.
Abstract
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.

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Proteins evolve on the edge of supramolecular self-assembly

TL;DR: Point mutations can frequently trigger folded proteins to self-assemble into higher-order structures and this potential is counterbalanced by negative selection and can be exploited to design nanomaterials in living cells.
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Aptamer-based lateral flow assay for point of care cortisol detection in sweat

TL;DR: The resulting LFA aptasensor provides a rapid, sensitive, user-friendly and cost-effective point of care device for cortisol detection in sweat and other biofluids.
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A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein.

TL;DR: A new class of FP from an allophycocyanin α-subunit that covalently attaches a biliverdin chromophore without a lyase, and has 642/670-nm excitation–emission peaks, a large extinction coefficient, and photostability comparable to that of eGFP.
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Warming-Induced Shrub Expansion and Lichen Decline in the Western Canadian Arctic

TL;DR: In this article, the authors present evidence that widespread and rapid shrub expansion and lichen declines over a 15,000 km2 area of the western Canadian Arctic have been driven by regional increases in temperature.
References
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Journal ArticleDOI

Fiji: an open-source platform for biological-image analysis

TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes

TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
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ImageJ for microscopy

TL;DR: The near-comprehensive range of import filters that allow easy access to image and meta-data, a broad suite processing and analysis routine, and enthusiastic support from a friendly mailing list are invaluable for all microscopy labs and facilities-not just those on a budget.
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Computer control of microscopes using µManager.

TL;DR: This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.
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Icy: an open bioimage informatics platform for extended reproducible research

TL;DR: Icy is a collaborative bioimage informatics platform that combines a community website for contributing and sharing tools and material, and software with a high-end visual programming framework for seamless development of sophisticated imaging workflows.
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