NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
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Cryo-EM shows how dynactin recruits two dyneins for faster movement.
Linas Urnavicius,Clinton K. Lau,Mohamed M. Elshenawy,Edgar Morales-Ríos,Carina Motz,Ahmet Yildiz,Andrew P. Carter +6 more
TL;DR: Cryo-electron microscopy reconstruction of a dynein tail–dynactin–BICDR1 complex reveals how dynactin can act as a scaffold to coordinate two dyneins side-by-side.
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D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction.
Sophie M. C. Gobeil,Katarzyna Janowska,Shana McDowell,Katayoun Mansouri,Robert Parks,Kartik Manne,Victoria Stalls,Megan Kopp,Rory Henderson,Rory Henderson,Robert J. Edwards,Robert J. Edwards,Barton F. Haynes,Barton F. Haynes,Priyamvada Acharya,Priyamvada Acharya +15 more
TL;DR: In this article, the effects of the D614G mutation on a soluble S ectodomain construct were explored and the results elucidate SARS-CoV-2 conformational landscape and allostery and have implications for vaccine design.
Journal ArticleDOI
Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh.
Aurélien Rizk,Gregory Paul,Pietro Incardona,Milica Bugarski,Maysam Mansouri,Axel Niemann,Urs Ziegler,Philipp Berger,Ivo F. Sbalzarini +8 more
TL;DR: A versatile protocol for a method named 'Squassh' (segmentation and quantification of subcellular shapes), which is used for detecting, delineating and quantifyingSubcellular structures in fluorescence microscopy images, implemented in freely available, user-friendly software.
Journal ArticleDOI
Loss of Junctional Adhesion Molecule A Promotes Severe Steatohepatitis in Mice on a Diet High in Saturated Fat, Fructose, and Cholesterol
Khalidur Rahman,Khalidur Rahman,Chirayu Desai,Smita S. Iyer,Natalie E. Thorn,Pradeep Kumar,Yunshan Liu,Tekla Smith,Tekla Smith,Andrew S. Neish,Hongliang Li,Shiyun Tan,Pengbo Wu,Xiaoxiong Liu,Yuanjie Yu,Alton B. Farris,Asma Nusrat,Charles A. Parkos,Frank A. Anania,Frank A. Anania +19 more
TL;DR: Findings indicate that intestinal epithelial barrier function and microbial dysbiosis contribute to the development of NASH.
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Genomics of Rapid Incipient Speciation in Sympatric Threespine Stickleback.
David Alexander Marques,David Alexander Marques,Kay Lucek,Kay Lucek,Kay Lucek,Joana I. Meier,Joana I. Meier,Salome Mwaiko,Salome Mwaiko,Catherine E. Wagner,Catherine E. Wagner,Catherine E. Wagner,Laurent Excoffier,Laurent Excoffier,Ole Seehausen,Ole Seehausen +15 more
TL;DR: It is suggested that adaptive genomic differentiation at many genetic loci can arise and persist in sympatry at the very early stage of ecotype divergence, and that the genomic architecture of adaptation may facilitate this.
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