NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
Citations
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STING-associated vasculopathy develops independently of IRF3 in mice
James D. Warner,Ricardo A. Irizarry-Caro,Brock G. Bennion,Teresa L. Ai,Amber M. Smith,Cathrine A. Miner,Tomomi Sakai,Vijay K. Gonugunta,Jianjun Wu,Derek J. Platt,Nan Yan,Jonathan J. Miner +11 more
TL;DR: The SAVI-associated STING N153S mutation triggers IRF3-independent immune cell dysregulation and lung disease in mice and causes myeloid cell expansion, T cell cytopenia, and dysregulation of immune cell signaling.
Journal ArticleDOI
Neuronal Activity and Glutamate Uptake Decrease Mitochondrial Mobility in Astrocytes and Position Mitochondria Near Glutamate Transporters
Joshua G. Jackson,John C. O'Donnell,Hajime Takano,Douglas A. Coulter,Douglas A. Coulter,Michael B. Robinson +5 more
TL;DR: Mitochondrial movement in astrocytes was inhibited by vinblastine and cytochalasin D, demonstrating that this mobility depends on both the microtubule and actin cytoskeletons, and suggesting a mechanism by which glutamate transporters might retain mitochondria at sites of glutamate uptake.
Journal ArticleDOI
IL13 activates autophagy to regulate secretion in airway epithelial cells
John D. Dickinson,Yael G. Alevy,Nicole P. Malvin,Khushbu Patel,Sean P. Gunsten,Michael J. Holtzman,Thaddeus S. Stappenbeck,Steven L. Brody +7 more
TL;DR: It is indicated that autophagy is essential for airway mucus secretion in a type 2, IL13-dependent immune disease process and thereby provide a novel therapeutic strategy for attenuating airway obstruction in hypersecretory inflammatory diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis lung disease.
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One-step purification of assembly-competent tubulin from diverse eukaryotic sources
Per O. Widlund,Marija Podolski,Simone Reber,Joshua Alper,Marko Storch,Anthony A. Hyman,Jonathon Howard,David N. Drechsel +7 more
TL;DR: A method is presented that allows rapid and efficient purification of native, active tubulin from a variety of species and tissue sources by affinity chromatography, which eliminates the need to use heterologous systems for the study of microtubule-associated proteins and motor proteins.
Journal ArticleDOI
Fourier ptychography: current applications and future promises
Pavan Chandra Konda,Lars Loetgering,Kevin C. Zhou,Shiqi Xu,Andrew R. Harvey,Roarke Horstmeyer +5 more
TL;DR: This review presents details about a relatively new procedure termed Fourier ptychography (FP), which addresses the above trade-off to produce gigapixel-scale images without requiring any moving parts.
References
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