NIH Image to ImageJ: 25 years of image analysis
TLDR
The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.Abstract:
For the past 25 years NIH Image and ImageJ software have been pioneers as open tools for the analysis of scientific images. We discuss the origins, challenges and solutions of these two programs, and how their history can serve to advise and inform other software projects.read more
Citations
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Pluripotent state transitions coordinate morphogenesis in mouse and human embryos
Marta N. Shahbazi,Antonio Scialdone,Natalia Skorupska,Antonia Weberling,Gaëlle Recher,Meng Zhu,Agnieszka Jedrusik,Liani Devito,Laila Noli,Iain C. Macaulay,Christa Buecker,Y. Khalaf,Dusko Ilic,Thierry Voet,Thierry Voet,John C. Marioni,John C. Marioni,John C. Marioni,Magdalena Zernicka-Goetz +18 more
TL;DR: It is shown that exit from an unrestricted naive pluripotent state is required for epiblast epithelialization and generation of the pro-amniotic cavity in mouse embryos and the relevance of transitions between these states during development of the mammalian embryo is shown.
Journal ArticleDOI
Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons
Matthew Redmann,Gloria A. Benavides,Taylor F. Berryhill,Willayat Yousuf Wani,Xiaosen Ouyang,Michelle S. Johnson,Saranya Ravi,Stephen Barnes,Victor M. Darley-Usmar,Jianhua Zhang +9 more
TL;DR: A significant impact of bafilomycin and chloroquine on cellular bioenergetics and metabolism consistent with decreased mitochondrial quality associated with inhibition of autophagy is demonstrated.
Journal ArticleDOI
Femtosecond laser surface texturing of titanium as a method to reduce the adhesion of Staphylococcus aureus and biofilm formation
Alexandre Cunha,Alexandre Cunha,Anne-Marie Elie,Laurent Plawinski,Ana Paula Serro,Ana M. Botelho do Rego,Amélia Almeida,Urdaci Maria Camino,Marie-Christine Durrieu,Rui Vilar +9 more
TL;DR: In this paper, the authors investigated the possibility of using femtosecond laser surface texturing as a method to reduce the colonization of Titanium alloy surfaces by Staphylococcus aureus and the subsequent formation of biofilm.
Journal ArticleDOI
MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosis
Andre L. Samson,Andre L. Samson,Ying Zhang,Ying Zhang,Niall D. Geoghegan,Niall D. Geoghegan,Xavier J Gavin,Katherine A Davies,Katherine A Davies,Michael J. Mlodzianoski,Michael J. Mlodzianoski,Lachlan Whitehead,Lachlan Whitehead,Daniel Frank,Daniel Frank,Sarah E Garnish,Sarah E Garnish,Cheree Fitzgibbon,Anne Hempel,Samuel N. Young,Annette V. Jacobsen,Annette V. Jacobsen,Wayne Cawthorne,Wayne Cawthorne,Emma J. Petrie,Emma J. Petrie,Maree C. Faux,Maree C. Faux,Kristy Shield-Artin,Kristy Shield-Artin,Najoua Lalaoui,Najoua Lalaoui,Joanne M Hildebrand,Joanne M Hildebrand,John Silke,John Silke,Kelly L. Rogers,Kelly L. Rogers,Guillaume Lessene,Guillaume Lessene,Edwin D. Hawkins,Edwin D. Hawkins,James M. Murphy,James M. Murphy +43 more
TL;DR: It is shown that MLKL trafficking and plasma membrane accumulation are crucial necroptosis checkpoints, and that accumulation of phosphorylatedMLKL at intercellular junctions promotes necroPTosis.
Journal ArticleDOI
An analysis of critical factors for quantitative immunoblotting.
TL;DR: This study implemented diagnostic experiments that assess an immunoblot-analysis workflow for accuracy and precision and showed that ignoring such diagnostics can lead to pseudoquantitative Immunoblot data that markedly overestimate or underestimate true differences in protein abundance.
References
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Johannes Schindelin,Ignacio Arganda-Carreras,Erwin Frise,Verena Kaynig,Mark Longair,Tobias Pietzsch,Stephan Preibisch,Curtis Rueden,Stephan Saalfeld,Benjamin Schmid,Jean-Yves Tinevez,Daniel J. White,Volker Hartenstein,Kevin W. Eliceiri,Pavel Tomancak,Albert Cardona +15 more
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Fabrice de Chaumont,Stephane Dallongeville,Nicolas Chenouard,Nicolas Hervé,Sorin Pop,Thomas Provoost,Vannary Meas-Yedid,Praveen Pankajakshan,Timothee Lecomte,Yoann Le Montagner,Thibault Lagache,Alexandre Dufour,Jean-Christophe Olivo-Marin +12 more
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